出芽短梗霉发酵产liamocin的培养条件研究  

Fermentative production of liamocin by Aureobasidium pullulans

作　　者：David Machaku 周丹凤 林琳 司振军 刘士旺[1,3] 魏培莲[1,3] 

机构地区：[1]浙江科技学院生物与化学工程学院,杭州310023 [2]浙江大学生物工程研究所,杭州310027 [3]浙江省农产品化学与生物加工技术重点实验室,杭州310023

出　　处：《浙江科技学院学报》2017年第6期414-418,共5页Journal of Zhejiang University of Science and Technology

基　　金：浙江省自然科学基金项目(LY15C200014)

摘　　要：liamocin是出芽短梗霉(Aureobasidium pullulans)的某些菌株在发酵过程中分泌的一种多元醇酯,现已证明其具有抗肿瘤、抗菌、表面活性剂等多种作用。我们筛选获得一株出芽短梗霉liamocin高产菌株,对其产liamocin的发酵条件进行初步研究,并建立了一种滤纸片法测定liamocin含量的简便方法。研究结果显示:菌株以葡萄糖、蔗糖、木糖作碳源,都可以有效产生liamocin;酵母提取物、硝酸钠、玉米浆是比较适合的氮源;发酵过程中培养基pH值变化较大,培养基初始pH值为48时,liamocin都可以较好地产出;培养基装量对liamocin产量的影响较大。该研究结果可为liamocin发酵条件的进一步优化提供参考。Liamocin,a kind of polyol lipid produced by some strains of Aureobasidium pullulans during fermentation,has been proved to have anticancer activity,antibacterial activity and can function as biosurfactant.The research screened out a high-producing strain of liamocin,conducted a preliminary study on media and culture conditions for liamocin production,and developed a simple method for determining the amount of liamocin by filter paper.The resultsshowed that glucose,sucrose and xylose were efficient carbon sources,and yeast extract,NaNO3 and corn steep liquor were efficient nitrogen sources for liamocin production.During the fermentation,pH value of the media changed greatly,with the initial pH of media ranged from4 to 8,liamocin could be efficiently produced.Moreover,the filling volume of the media affected the production of liamocin greatly.This study has paved a foundation for further fermentation optimization of liamocin fermentation conditions.

关 键 词：出芽短梗霉 重油 liamocin 发酵 

分 类 号：Q93-335[生物学—微生物学]