杏鲍菇重组漆酶对不同酚类污染物的降解研究  

Phenic pollution treatment using recombinant laccase from Pleurotus eryngii

作　　者：夏文静[1] 周学[1] 周燕[1] 李飞[1] 

机构地区：[1]南京师范大学泰州学院,江苏泰州225300

出　　处：《食品工业科技》2017年第24期99-103,共5页Science and Technology of Food Industry

基　　金：江苏省高校自然科学研究面上项目(15KJB220004);江苏省高校自然科学研究面上项目(16KJB180017);泰州市社会发展项目(TS201516);南京师范大学泰州学院第二批精品课程<酶工程>

摘　　要：探讨重组漆酶的酶学性质,并研究降解时间、酶用量、酚类物质浓度、pH对两种不同结构酚类化合物(壬基酚、2,4-二氯酚)的降解效果。结果表明,该重组漆酶的最适反应温度为60℃,最适反应pH为2.5~3.0;该酶在30℃下具有较好的热稳定性,在pH9.0~10.0具有较好的pH稳定性。该重组漆酶具有降解不同结构酚类的能力,漆酶对不同浓度酚类化合物的降解率随着酚类浓度的增加而减小。当降解时间为16 h、重组漆酶浓度1 U/m L、pH3.5时,10 mg/L的壬基酚的降解率为100%;当降解时间为16 h、漆酶浓度是1.25 U/m L、pH4.0时,5 mg/L的2,4-二氯酚的降解率为95.1%。该重组漆酶的研究为酚类污染物的降解提供了理论基础。The enzymatic properties of recombined laccase derived from Pleurotus eryngii were investigated. The degradation effects of laccase on two kinds of phenols （ nonylphenol and 2,4 - dichlorophen ） were evaluated through optimizing the degradation time, enzyme dosage, phenol concentration and pH. The optimal reaction temperature of recombined lacease was 60℃ ,while the optimal pH was 2.5-3.0.The recombined laccase could keep high thermal stability below 30 ℃ and high pH stability between 9.0N 10.0.The recombined lacease could decompose phenols with different structures, but the degradation ratios of phenols by laccase were decreased with increasing concentrations of phenols.With degradation time of of 16 h, enzyme dosage of 1 U/mL,pH of 3.5 ,the degradation ratio of nonylphenol（ 10 mg/L） reached up to 100% ;with degradation time of 16 h, enzyme dosage of 1.25 U/mL, pH of 4.0, the degradation ratio of 2,4-dichlorophen （5 mg/L）reached up to 95.1% .The study of recombined laccase derived from Pleurotus eryngii indicated a theoretical basis for the treatment of phenol- containing pollution.

关 键 词：重组漆酶 壬基酚 2 4-二氯酚 降解 

分 类 号：TS201.3[轻工技术与工程—食品科学]