ⅢA-N2期非小细胞肺癌组织TYMS基因mRNA表达与EGFR基因突变的相关性研究  

Study on correlation between TYMS gene mRNA expression and EGFR gene mutation in stage ⅢA-N2 non-small-cell lung cancer tissue

作　　者：李喆[1] 杨扬[1] 刘延风[1] 许瑞彬[1] 戎铁华[2] 张兰军[2] 

机构地区：[1]延安大学附属医院胸外科,陕西延安716000 [2]中山大学肿瘤防治中心胸科/华南肿瘤学国家重点实验室,广州510060

出　　处：《重庆医学》2017年第35期4977-4979,共3页Chongqing medicine

摘　　要：目的探讨ⅢA-N2期非小细胞肺癌(NSCLC)组织胸苷酸合成酶(TYMS)基因mRNA表达与表皮生长因子受体(EGFR)基因突变的相关性。方法分别用分支DNA-液相芯片法及突变富集-液相芯片法检测30例病理分期为ⅢA-N2期的NSCLC组织TYMS mRNA表达及EGFR基因19号及21号外显子突变状况,并对检测结果进行整理分析。结果 30例患者中,TYMS mRNA低表达14例(46.7%),中偏低表达7例(23.3%),中表达7例(23.3%),中偏高表达0例,高表达2例(6.7%);12例检出EGFR基因突变,突变率为40.0%,其中6例为19外显子缺失突变,6例为21外显子置换突变。TYMS mRNA表达水平与EGFR突变相关,EGFR突变多发生在TYMS mRNA较低表达水平的患者肿瘤组织中(Z=-2.604,P=0.009)。结论NSCLC组织中TYMS mRNA表达与EGFR基因突变相关,可为今后临床中针对不同条件的患者相关药物的选用提供参考。Objective To investigate the correlation between TYMS gene mRNA expi＇ession level and EGFR mutation in stage Ⅲ A-N2 non-small：celt lung cancer tissue （NSCLC）. Methods The branched DNA-liquidchip technology （bDNA-LCT） and mutant-enriched tiquidchip （MEL） technology were used to detect the TYMS mRNA expression and EGFR mutations at exon 19 and 21 in NSCLC tissues from 30 patients with stages Ⅲ A-N2 NSCLC, and the results were analyzed. Results 30 cases, low TYMS mRNA expression was in 14 cases （46. 7%） ,middle to low TYMS mRNA expression in 7 cases （23.3%） ,middle mR- NA expression in 7 cases （23. 3 % ）, middle to high TYMS mRNA expression in 0 case and high TYMS mRNA expression in 2 cases （6.7%） ;12 cases of EGFR mutation were detected out with the mutation rate of 40.0% ,including 6 cases of exon 19 deletion and 6 cases of exon 21 missense mutation. The TYMS mRNA expression level was correlated with the EGFR mutation. The EGFR mutation commonly occurred in the tumor tissue of the patients with TYMS rdRNA low expression （Z= -2. 604, P=0. 009）. Conclusion The TYMS mRNA expression in NSCLC tissue is correlated with the EGFR gene mutation,which can provide references for the drug selection aiming at the patients with different conditions.

关 键 词：癌 非小细胞肺 胸苷酸合成酶 受体 表皮生长因子 基因表达 基因突变 

分 类 号：R734.2[医药卫生—肿瘤]