川陈皮素对高脂膳食诱导大鼠的降脂减肥及预防脂肪肝形成作用  

Hypolipidemic and Anti-Obesity Effect of Nobiletin and Its Role in Prevention of Fatty Liver in High Fat Diet-Fed Rats

作　　者：冯孔龙 朱晓艾[1,2] 陈彤 彭勃[1,2] 张立群 戴伟杰[1,2] 刘欣 曹庸[1,2] 陈运娇 FENG Konglong;ZHU Xiaoai;CHEN Tong;PENG Bo;ZHANG Liqun;DAI Weijie;LIU Xin;CAO Yong;CHEN Yunjiao(College of Food Science, South China Agricultural University, Guangzhou 510642, China;Guangdong Engineering Research Center of Natural Active Substance, Guangzhou 510642, China;College of Fundamental Medical Science, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China)

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]广东省天然活性物工程技术研究中心,广东广州510642 [3]广州中医药大学基础医学院,广东广州510006

出　　处：《食品科学》2018年第1期213-220,共8页Food Science

基　　金：广东省天然活性物工程技术研究中心项目(2016B090920093);广东省教育厅科研项目(2013gjhz0003);广东省自然科学基金博士启动项目(2015A030310118)

摘　　要：目的:研究川陈皮素对高脂膳食诱导的大鼠体质量、血脂和肝脏的影响。方法:将56只雄性SD大鼠随机分为7组,即正常组、高脂对照组、阳性对照组(奥利司他和力平之)和川陈皮素低、中、高剂量组(高脂饲料+0.02%、0.04%、0.08%川陈皮素),对正常组饲喂普通饲料,其余各组分别饲喂相应的高脂饲料,连续喂养6周后,考察不同时期大鼠血清血脂水平变化,检测大鼠体质量、体内脂肪质量、脏器变化及肝脏组织形态变化。结果:川陈皮素可以有效抑制高脂膳食大鼠的体质量增长,降低Lee’s指数、食物利用率和脂肪系数;降低血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)和低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)水平,其中降低TG作用显效较快,降低血清TC和LDL-C作用需要较长时间;同时可降低肝脏指数、血清中谷草转氨酶和谷丙转氨酶水平,改善肝脏脂肪变性情况。结论:川陈皮素具有预防肥胖、辅助降血脂及预防肝脏脂肪变性的作用。Objective： The effect of nobiletin on the body weight, serum lipids and liver of rats exposed to a high-fat diet were investigated. Methods： Totally 56 male SD rats were randomly divided into seven groups： normal control group, high-fat diet control group, positive control groups （orlistat and fenofbrate）, and low-, middle- and high-dose nobiletin groups （high-fat diet ＋ 0.02%、0.04%、0.08% nobiletin）. The rats in the normal control group were fed normal diet, while the remaining groups were fed high-fat diet plus the corresponding drugs for six weeks. Then serum lipid parameters, body weight, body fat weight, organ weight to body weight ratios and liver histopathology were examined. Results： Nobiletin could inhibit body weight gain induced by high-fat diet in rats, reduce Lee’s index, food utilization rate and fat to body weight ratio, and decrease serum total cholesterol （TC）, triglyceride （TG）, and low-density lipoprotein cholesterol （LDL-C） levels with the fastest rate of decrease being observed for TG. Compared with the high-fat control group, liver index and serum glutamic oxalacetic transaminase （AST） and alanine transaminase （ALT） activities in the nobiletin treatment groups were decreased, and fatty degeneration of the liver was improved. Conclusion： Nobiletin can reduce serum lipids and prevent obesity and fatty liver.

关 键 词：川陈皮素 高脂膳食 大鼠 预防肥胖 降血脂 预防脂肪肝 

分 类 号：R151.1[医药卫生—营养与食品卫生学]