响应面分析优化L-色氨酸清液发酵培养基  

Culture medium optimization for L-tryptophan cleaned liquid fermentation by response surface analysis

作　　者：杨梦晨 户红通[1,2] 刘子强 陈宁 徐庆阳 

机构地区：[1]天津科技大学生物工程学院/代谢控制发酵技术国家地方联合工程实验室,天津300457 [2]天津科技大学,天津市氨基酸高效绿色制造工程实验室,天津300457

出　　处：《生物资源》2017年第6期464-471,共8页Biotic Resources

基　　金：天津市科技支撑计划重点项目(14ZCZDSY00015);天津市科技特派员项目(15JCTPJC57000)

摘　　要：L-色氨酸是八种必需氨基酸之一,随着L-色氨酸应用市场的不断扩大,进行发酵法生产L-色氨酸的研究具有重要的现实意义。为了提高L-色氨酸产量,本文利用响应面分析法对L-色氨酸清液发酵培养基进行优化。利用优化培养基进行发酵,考察清液发酵对L-色氨酸发酵过程中生物量、L-色氨酸产量、副产物生成量的影响。结果表明:在优化条件下利用清液发酵培养基发酵,乙酸含量与原工艺相比降低了(6.75±1.26)%,L-色氨酸产量提高了(16.54±1.15)%,实验值与响应面分析预测值基本相符。L-tryptophan is an essential amino acid, and the work for L-tryptophan production by microbial fermen tation has important practical siginificance with the development of L tryptophan market. In this study, the fermentation medium was optimized by response surface analysis, and the effects of cleaned liquid fermentation on biomass and L-tryptophan yield of Escherichia coli TRTH in the fermentation process were observed. Under the optimum fermentation medium for fermentation, acetic acid concentration decreased by （6.75± 1.26）% compared with the original process while the L-tryptophan production increased by （16.54 ± 1.15） %. The results showed that the experimental values agreed with the predicted values,

关 键 词：L-色氨酸 大肠杆菌 清液发酵 响应面分析 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]