鱼油对高脂喂养加小剂量STZ诱导的2型糖尿病大鼠血脂及肝脏的影响  

Effect of Fish Oil on the Lipid Metabolism of Liverin High Fat Diet Plus Low-dose STZ induced Type 2 Diabetic Rats

作　　者：王颖[1] 戚瑞[1] 张磊[1] 王晓慧[1] 徐孝娜[1] 谢祥军[1] 杨瑞华[1] 

机构地区：[1]第四军医大学军事预防医学系营养与食品卫生学教研室,陕西西安710032

出　　处：《现代生物医学进展》2017年第33期6406-6411,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81271194);陕西省自然科学基金项目(2011JM4002)

摘　　要：目的:探讨鱼油对高脂喂养加小剂量STZ诱导的2型糖尿病大鼠脂代谢及肝脏组织的影响及其机制。方法:Sprague-Dawley大鼠随机分为对照组(Control)、高脂组(HF)、糖尿病组(DM)、高脂鱼油组(HF+FO)和糖尿病鱼油组(DM+FO),观察大鼠周体重变化,检测各组大鼠血糖、血脂、肝脏细胞形态学及过氧化损伤情况,探讨其可能的作用机制。结果:与对照组相比,高脂喂养大鼠体重明显增加(387.74±36.20 g vs 339.64±10.41 g,P<0.05),血甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)和低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)分别由1.96±0.06 mmol/L、1.91±0.09 mmol/L和0.72±0.07 mmol/L升高至2.29±0.16 mmol/L、2.08±0.06 mmol/L和1.22±0.09 mmol/L,高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)降低(0.92±0.15 mmol/L vs 1.80±0.07 mmol/L,P<0.05),膳食添加鱼油后均有明显改善。糖尿病大鼠体重减轻(287.78±16.11 g vs 339.64±10.41 g,P<0.05);但血清中LDL-C水平升高至1.08±0.08 mmol/L,HDL-C水平降低至1.09±0.07 mmol/L(P<0.05),鱼油可显著提高HDL-C的水平(P<0.05)。高脂组和糖尿病组均有明显的肝脏脂肪浸润和过氧化损伤,添加鱼油后明显减轻。结论:膳食添加鱼油对高脂膳食及2型糖尿病引起的血脂异常均有保护作用,可降低血中甘油三酯和低密度脂蛋白胆固醇水平;减轻大鼠肝脏脂肪浸润和过氧化损伤。Objective： To explore the effect of fish oil on the lipid metabolism and liver injury of high fat diet and low-dose strep- tozotocin （STZ） induced type 2 diabetic rats. Methods： Spragne-Dawley rats were randomly divided into control group （Control）, high fat group （HF）, diabetes mellitus group （DM）, high fat ＋ fish oil group （HF ＋ FO） and diabetes mellitus ＋ fish oil group （DM ＋ FO）. Body weight changes were detected weekly, and blood sugar, blood fat, liver cell morphological changes and hyperoxidative injury were de- tected at the beginning and end of the experimental period. Further explore the mechanisms of injury and effect offish oil. Results： Com- paring with control group, body weight of high fat diet rats increased （387.74± 36.20 g vs 339.64± 10.41 g, P〈0.05）. High fat diet in- duced triacylglyceride （TG）, total cholesterol （TC） and low density lipoprotein-cholesterol （LDL-C） increase from 1.96±0.06 mmol/L, 1.91± 0.09 mmol/L and 0.72± 0.07 mmol/L to 2.29±0.16 mmol/L, 2.08±0.06 mmol/L and 1.22＋ 0.09 mmol/L, respec- tively. High density lipoprotein-cholesterol （HDL-C） decrease in rats（0.92±0.15 mmol/L vs 1.80±0.07 retool/L, P〈0.05 ）. Dietary fish oil reversed the changes significantly. The body weight of diabetic rats decreased（287.78± 16.11 g vs 339.64± 10.41 g, P〈0.05）. The serum level of LDL-C increased to 1.08±0.08 mmoFL, and HDL-C decreasedto 1.09± 0.07 mmol/L （P〈0.05）. Fish oil supplementation increased HDL-C in diabetic rats （P〈0.05）. Fatty infiltration and peroxidative injury of liver were observed in both HF and DM rats, and dietary fish oil improved the injury. Conclusion： Dietary fish oil has protective effect on hyperlipidemia in high fat diet and type 2 diabetes melli- tus rats. It can decrease blood TG and LDL-C level and reduce the fatty infiltration and oxidative injury of liver.

关 键 词：高脂饮食 脂代谢 2型糖尿病 鱼油 氧化应激 

分 类 号：R33[医药卫生—人体生理学] R587.2[医药卫生—基础医学]