法国蓝薰衣草挥发油改善记忆障碍的药效评价研究  

Research on improving memory impairment of blue lavender volatile oil

作　　者：朱丽云[1,2] 高永生[1,2] 宋林珍[1,2] 李素芳[1,2] 钱俊青 

机构地区：[1]中国计量大学芳香工程技术研发中心,浙江杭州310018 [2]中国计量大学海洋食品加工质量控制技术与仪器国家地方联合工程实验室,浙江杭州310018 [3]浙江工业大学生物工程学院,浙江杭州310014

出　　处：《中国中药杂志》2017年第24期4819-4826,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(31571845);国际合作项目(40-16);浙江省重点科技创新团队项目(2010R50028)

摘　　要：为研究薰衣草挥发油改善记忆障碍的潜在应用价值,采用GC-MS技术分析了薰衣草挥发油的化学组成,并建立小鼠阿尔茨海默病(Alzheimer's disease,AD)模型,以跳台实验和脑组织中T-SOD,GSH-PX,CAT等抗氧化酶活性及其产物MDA含量的变化等研究了薰衣草挥发油的改善记忆障碍功能。实验结果表明,GC-MS技术分析鉴定了薰衣草挥发油55种化学成分,主要为萜烯、萜醇和酯类化合物,樟醇和乙酸芳樟酯含量最高,占总挥发油的49.71%。薰衣草挥发油给药组均显著改善了小鼠跳台记忆能力,对小鼠脑组织中GSH-PX、CAT和T-SOD水平均显著高于模型组(P<0.05),MDA在模型组中达到最高,药物组MDA则与正常组水平无差异,体现了薰衣草挥发油显著的抗脑组织氧化活性,存在诱导神经元氧化应激减少的可能性,使记忆获得性障碍出现扭转的作用。In order to study the potential application value of lavender volatile oil （LVO）, the chemical composition of the volatile oil of lavender was analyzed by GC-MS, and the mouse model of Alzheimer＇s disease （AD） was established. Additionally, the antioxidant enzymes activity of T-SOD, GSH-PX, CAT and MDA content were studied. Experimental results showed that 55 kinds of chemical constituents including terpene, terpene alcohol and ester compounds from LVO were identified, and the content of linalool and linalyl acetate was the highest, accounting for 49.71% of the total volatile oil. The ability of mouse platform memory was improved significantly. The levels of GSH-PX, CAT and T-SOD of mouse brain tissue in the treatment group were significantly higher than those in the model group （P〈0.05）. The level of MDA reached the maximum value in the model group, while there was no notable difference between the levels of MDA in the drug group and the normal group. The result indicated the significant oxidative activity of LVO, the possibility of induced oxidative stress reduction in neurons, and the reversal effect of memory acquired disorder.

关 键 词：薰衣草挥发油 记忆障碍 成分分析 抗氧化酶 

分 类 号：R284[医药卫生—中药学] R285.5[医药卫生—中医学]