白茶实时荧光定量PCR分析中内参基因的筛选与验证  

Selection and Validation of Reference Genes for Real-time Florescence Quantitative PCR Analysis on Gene Expression of White Tea

作　　者：陈静[1,2] 郑知临 林浥 曹红利 陈笛[1] 陈桂信 叶乃兴[1] 

机构地区：[1]福建农林大学园艺学院/茶学福建省高校重点实验室,福建福州350002 [2]宁德职业技术学院生物技术系,福建福安355000

出　　处：《福建农业学报》2017年第11期1201-1206,共6页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31270735);福建农林大学科技创新专项(CXZX2016117;CXZX2017313)

摘　　要：利用GeNorm,NormFinder,BestKeeper软件对茶树不同叶位及白茶萎凋过程中7个候选内参基因的表达稳定性进行分析。获得3个稳定性较好的内参基因β-Actin、GAPDH和RUBP,RUBP适合作为白茶萎凋过程叶片荧光定量PCR的内参基因,β-Actin适合作为白茶不同叶位叶片荧光定量PCR的最佳内参基因。因此,在白茶萎凋过程中,使用GAPDH+RUBP组合作为内参基因进行荧光定量PCR检测。To select appropriate reference genes to be used in the real-time florescence quantitative PCR analysis for studying the gene expression in of white tea leaves during withering, the software of GeNorm, NormFinder, and BestKeeper were applied. Three stable reference genes, β-Actin, GAPDH and RUBP, were identified. Among them, RUBP was found most adequate for comparing the expressions at various stages of withering, while β-Actin for differentiating them in leaves from different parts of a tea plant. The combination of GAPDH＋RUBP was determined as choice reference genes for the analysis.

关 键 词：白茶 萎凋 内参基因 表达稳定性 荧光定量PCR 

分 类 号：S571[农业科学—作物学]