异源表达细菌β-甘露聚糖酶研究进展  

Heterologous Expression Bacterial β-mannanase: Research Progress

作　　者：那金[1,2] 王瑶 郭尚旭[1,2] 赵丹 

机构地区：[1]黑龙江大学生命科学学院微生物省高校重点实验室,哈尔滨150080 [2]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150500

出　　处：《农学学报》2017年第12期18-21,27,共5页Journal of Agriculture

基　　金：哈尔滨市科技局青年后备人才项目"功能性乳酸菌发酵酸菜生态学过程研究"(2014RFQXJ101);黑龙江省政府博士后项目"疣孢漆斑菌(Myrothecium verrucaria)NF-05漆酶的产生;特性及应用研究"(LBH-Z15214)

摘　　要：甘露聚糖酶是一类水解甘露聚糖的关键酶,广泛存在于动植物和微生物中,细菌来源尤为广泛。为了更好地满足工业生产的需求,异源表达细菌来源的甘露聚糖酶基因,以获得性质优良的甘露聚糖酶。对甘露聚糖酶的来源和酶学性质进行了概述,归纳了细菌甘露聚糖酶的异源表达在提高酶活、扩大p H值作用范围、改善安全性等方面的作用,包括对埃希氏大肠杆菌、枯草芽孢杆菌及毕赤酵母进行异源表达等。利用微生物资源拓宽甘露聚糖酶的应用领域,使用微生物技术提高产酶量满足工业需求,并为甘露聚糖酶定向进化和改造奠定基础。Mannanase is a key enzyme for hydrolyzing mannan, and is widely found in plants, animals andmicroorganisms, particularly in bacteria. In order to meet the needs of industrial production, heterologousexpression of bacterial-derived mannanase gene is studied in order to obtain high-quality mannanase. In thispaper, the origin and enzymatic properties of mannanase were reviewed, and the heterologous expression ofbacterial mannanase was summarized concerning the aspects of improving the enzyme activity, expanding thep H range and improving the safety, including heterologous expression of Escherichia coli, Bacillus subtilis and Pichia pastoris. The use of microbial resources can broaden the application of mannanase, and the use ofmicrobial technology can improve the amount of enzyme production to meet industrial needs, these lay afoundation for the determinate evolution and transformation of mannanase.

关 键 词：细菌 甘露聚糖酶 分子生物学 异源表达 应用 

分 类 号：TS2[轻工技术与工程—食品科学与工程] C39[社会学]