余甘子酚类成分及其抑制α-葡萄糖苷酶活性的研究  

Structural Identification of Phenolic Constituents from Phyllanthus emblica L. and Their Inhibitory Activity on α-glucosidase

作　　者：刘伟[1] 李明玺 王俊龙[1] 万春鹏 

机构地区：[1]伊犁师范学院,自治区教育厅普通高校重点实验室,新疆伊宁835000 [2]江西农业大学农学院,江西省果蔬保鲜与无损检测重点实验室/江西省果蔬采后处理关键技术与质量安全协同创新中心,江西南昌330045

出　　处：《现代食品科技》2017年第12期50-55,共6页Modern Food Science and Technology

基　　金：新疆维吾尔自治区教育厅普通高校重点实验室资助项目(2017YSHXZD01);国家自然科学基金青年科学基金项目(31500286)

摘　　要：研究余甘子甲醇提取物降糖活性部位中的酚类化学成分及其抑制α-葡萄糖苷酶活性。采用甲醇浸泡提取余甘子,D101大孔树脂柱粗分离得到三个部位,降糖活性测试表明30%乙醇洗脱部位(PEA)为活性部位,后续采用Sephadex LH-20,ODS和制备HPLC等柱色谱技术对其进行分离纯化,根据化合物理化性质、NMR和HR-ESI-MS数据并参考相关文献鉴定化合物结构,采用体外方法测定化合物抑制α-葡萄糖苷酶活性。从PEA部位中分离并鉴定了7个酚类化合物,分别为:没食子酸(1),没食子酸甲酯(2),1-O-没食子酰基-β-D-葡萄糖苷(3),1,6-二-O-没食子酰基-β-D-葡萄糖苷(4),柯里拉京(5),粘酸-1,4-内酯-2-O-没食子酸酯(6),粘酸-2-O-没食子酸酯(7)。除化合物2外所有化合物均具有一定的抑制α-葡萄糖苷酶活性。没食子酸衍生物是余甘子抑制α-葡萄糖苷酶活性成分。The phenolic constituents in the hypoglycemic active fraction of the methanol extract of Phyllanthus emblica L and their inhibitory activity on α-glucosidase were studied. Using methanol soaking extract Phyllanthus emblica L, the methanol extract（PE） was chromatographed on a D101 macroporous resin column to obtain three fractions（PEA-PEC）. Hypoglycemic activity test showed that 30% ethanol elution part（PEA） was the active fraction. PEA was further isolated and purified by column chromatography of Sephadex LH-20, ODS and semi-preparative HPLC columns. The structures of the isolated compounds were identified according to the physicochemical properties, 1 H-NMR, 13 C-NMR and high resolution mass spectral analyses as well as relevant references. The compounds were tested for their in vitro inhibitory activity on α-glucosidase. Seven phenolic compounds were isolated from PEA fraction and identified as gallic acid（1）, methyl gallate（2）, 1-O-galloyl-β-D-glucose（3）, 1,6-di-O-galloyl-β-D-glucose（4）, corilagin（5）, mucic acid-1,4-Lactone-2-O-gallate（6）, mucic acid-2-O-gallate（7）. All compounds except compound 2 showed good inhibitory activity on α-glucosidase. Gallic acid derivatives were the active constituents that P. emblica L. inhibited the activity of α-glucosidase.

关 键 词：余甘子 化学成分 酚类成分 α-葡萄糖苷酶抑制活性 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]