裙带菜乙醇洗脱组分对α-葡萄糖苷酶活性的影响  

Effects of Various Ethanol Elutions of Undaria Pinnatifida on α-glucosidase Activity

作　　者：黄晓冬 黄世英[1,2,3] 戴聪杰 韦兰妮 何嘉嘉[1] 王惠民 

机构地区：[1]泉州师范学院海洋与食品学院,福建泉州362000 [2]泉州师范学院福建省海洋藻类活性物质制备与功能开发重点实验室,福建泉州362000 [3]泉州师范学院近海资源生物技术福建省高校重点实验室,福建泉州362000 [4]中兴大学生医工程研究所,台湾台中40227

出　　处：《泉州师范学院学报》2017年第6期12-16,共5页Journal of Quanzhou Normal University

基　　金：泉州市科技计划项目(2014Z129);福建省(省级)大学生创新创业计划项目(201710399026)

摘　　要：探讨养殖型褐藻裙带菜水提醇沉上清部分洗脱组分对α-葡萄糖苷酶活性的抑制作用及其反应动力学.采用AB-8大孔吸附树脂柱,依次用去离子水、25%乙醇、50%乙醇、75%乙醇、100%乙醇梯度洗脱裙带菜水提醇沉上清部分,获得相应洗脱组分.采用PNPG法测定α-葡萄糖酶活性,并与阳性对照阿卡波糖作比较.随后,对其中抑制α-葡萄糖苷酶活性较强的组分进行酶抑制动力学分析,推断酶抑制类型.结果表明:相比于裙带菜水提醇沉上清部分与其他洗脱组分,50%乙醇洗脱组分具有较强的α-葡萄糖苷酶抑制活性,半效应浓度IC50为0.77mg/mL,远小于阿卡波糖IC506.63 mg/mL,其酶抑制类型属于混合I型抑制,主要通过与游离酶(E)的亲和来起到抑制作用.因此,裙带菜水提醇沉上清部分50%乙醇洗脱组分具有作为新型α-葡萄糖苷酶抑制剂材料的开发价值.This study aimed to explore the effects of various elutions from the supernatant from water extract-alcohol precipitation of Undaria pinnatifida onα-glucosidase activity and its reaction kinetics.For various elutions,the supernatant from water extract-alcohol precipitation of Undaria pinnatifida was eluted by the AB-8 macroporous resin with water,25%,50%,75%,and 100% ethanol,respectively.The effects of various fractions onα-glucosidase activity were determined by the PNPG method,and acarbose was used as a positive control.The elution fraction with the strongestα-glucosidase inhibitory activity was then selected for the enzymatic kinetic analysis and identifying its inhibitory type.The results showed that the 50%ethanol elution from the supernatant from water extract-alcohol precipitation of Undaria pinnatifida had strongestα-glucosidase inhibitory activity by comparing with the supernatant and other elution fractions.The IC_（50） value of the 50% ethanol elution was 0.77 mg/mL,which was much less than the IC50 of acarbose with 6.63 mg/mL.Enzyme inhibitory type of the 50% ethanol elution was mixed Ⅰtype inhibition,and its inhibitory effects were mainly through the affinity with free enzyme（E）.These results indicated that the 50%ethanol elution from the supernatant from water extract-alcohol precipitation of Undaria pinnatifida should have developmental value to be a source of newα-glucosidase inhibitor.

关 键 词：裙带菜 水提醇沉上清部分 乙醇洗脱组分 Α-葡萄糖苷酶活性 抑制动力学 

分 类 号：R931.71[医药卫生—生药学]