Interleukin-1βinduces human cementoblasts to support osteoclastogenesis  

作　　者：nam c-n huynh vincent everts prasit pavasant ruchanee s ampornaramveth 

机构地区：[1]research unit on oral microbiology and immunology,microbiology department,faculty of dentistry,chulalongkorn university,Bangkok,Thailand [2]mineralized tissue research unit,faculty of dentistry,chulalongkorn university,Bangkok,Thailand [3]department of dental basic sciences,faculty of odonto-stomatology,university of medicine and pharmacy at ho chi minh city,HO Chi Minh City,Vietnam [4]department of oral cell biology,academic centre for dentistry amsterdam(acta),university of amsterdam and vu university amsterdam,The Netherlands [5]department of anatomy,faculty of dentistry,chulalongkorn university,Bangkok,Thailand

出　　处：《International Journal of Oral Science》2017年第4期216-223,共8页国际口腔科学杂志（英文版）

基　　金：Ratchadaphiseksomphot Endowment Fund Chulalongkorn University(R/F_2557_021_03_32);Integrated-Innovation Academic Center’Chulalongkorn University Centenary Academic Development Project;The 2012 Research Chair Grant;Thailand National Science and Technology Development Agency(NSTDA)

摘　　要：Injury of the periodontium followed by inflammatory response often leads to mot resorption. Resorption is accomplished by osteoclasts and their generation may depend on an interaction with the cells in direct contact with the root, the cementoblasts. Our study aimed to investigate the role of human cementoblasts in the formation of osteoclasts and the effect of interleukin （IL）- 1β hereupon. Extracted teeth from healthy volunteers were subjected to sequential digestion by type I collagenase and trypsin. The effect of enzymatic digestion on the presence of cells on the root surface was analyzed by histology. Gene expression of primary human cementoblasts （pHCB） was compared with a human cementoblast cell line （HCEM）. The pHCBs were analyzed for their expression of IL-1 receptors as well as of receptor activator of nuclear factor kappa-B ligand （RANKL） and osteoprotegerin （OPG）. In a co-culture system consisting of osteoclast precursors （blood monocytes） and pHCBs, the formation of osteoclasts and their resorptive activity was assessed by osteo-assay and ivory slices. The cells obtained after a 120 min enzyme digestion expressed the highest level of bone sialoprotein, similar to that of HCEM. This fraction of isolated cells also shared a similar expression pattern of IL-1 receptors （ILl-R1 and ILl-R2）. Treatment with IL-11~ potently upregulated RANKL expression but not of OPG. pHCBs were shown to induce the formation of functional osteoclasts. This capacity was significantly stimulated by pretreating the pHCBs with IL-1β prior to their co-culture with human blood monocytes. Our study demonstrated that cementoblasts have the capacity to induce osteoclastogenesis, a capacity strongly promoted by IL-1β. These results may explain why osteoclasts can be formed next to the root of teeth.Injury of the periodontium followed by inflammatory response often leads to mot resorption. Resorption is accomplished by osteoclasts and their generation may depend on an interaction with the cells in direct contact with the root, the cementoblasts. Our study aimed to investigate the role of human cementoblasts in the formation of osteoclasts and the effect of interleukin （IL）- 1β hereupon. Extracted teeth from healthy volunteers were subjected to sequential digestion by type I collagenase and trypsin. The effect of enzymatic digestion on the presence of cells on the root surface was analyzed by histology. Gene expression of primary human cementoblasts （pHCB） was compared with a human cementoblast cell line （HCEM）. The pHCBs were analyzed for their expression of IL-1 receptors as well as of receptor activator of nuclear factor kappa-B ligand （RANKL） and osteoprotegerin （OPG）. In a co-culture system consisting of osteoclast precursors （blood monocytes） and pHCBs, the formation of osteoclasts and their resorptive activity was assessed by osteo-assay and ivory slices. The cells obtained after a 120 min enzyme digestion expressed the highest level of bone sialoprotein, similar to that of HCEM. This fraction of isolated cells also shared a similar expression pattern of IL-1 receptors （ILl-R1 and ILl-R2）. Treatment with IL-11~ potently upregulated RANKL expression but not of OPG. pHCBs were shown to induce the formation of functional osteoclasts. This capacity was significantly stimulated by pretreating the pHCBs with IL-1β prior to their co-culture with human blood monocytes. Our study demonstrated that cementoblasts have the capacity to induce osteoclastogenesis, a capacity strongly promoted by IL-1β. These results may explain why osteoclasts can be formed next to the root of teeth.

关 键 词：cementoblast interleukin-ll3 OSTEOCLAST receptor activator of nuclear factor kappa-B ligand tooth resorption 

分 类 号：R781.4[医药卫生—口腔医学]