响应面法优化小球藻多糖提取工艺研究  

Optimization of Extraction Technology of Polysaccharides from Chlorella by Response Surface Methodology

作　　者：史瑞琴 李大伟 梁静静 王颉[1] 马艳莉[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071000

出　　处：《食品研究与开发》2018年第3期18-23,共6页Food Research and Development

基　　金：河北省科技计划项目(15273204D);国家海洋公益性行业科研专项(201205031)

摘　　要：主要通过超声波对小球藻粉溶液进行预处理后,采用复合酶辅助热水浸提法提取小球藻多糖,并进行响应面法优化小球藻多糖的提取条件。经单因素及响应面试验结果分析表明,提取小球藻多糖的适宜条件为:液料比为20∶1(mL/g)、复合酶添加量1.45%、pH值5、酶解温度39℃,在此条件下小球藻多糖的提取率为6.56%。After the pretreatment of chlorella algae powder solution by ultrasonic method, the polysaccharide was extracted by hot water extraction with compound enzyme method, and the extraction conditions of polysac-charide were optimized by response surface methodology. By single factor test and response surface analysis, the results showed that the optimum extraction conditions were as follows： the ratio of solvent to materia was 20 ： 1 （mL/g ） , the amount of compound enzyme was 1.45 %, the pH value was 5, and the enzymolysis temper-ature was 39 Under this condition, the extraction rate of polysaccharides from chlorella was 6.56 %.

关 键 词：小球藻 S维素酶 木瓜蛋白酶 多糖 响应面法 

分 类 号：TQ281[化学工程—有机化工]