黒参粗多糖抑制人胃和肝癌细胞增殖作用的研究  

Study on human gastric carcinoma cell and human liver cancer cell inhibition rates of grude polysaccharides derived from black ginseng

作　　者：刘佳维[1] 石秀梅[1] 于凤波[1] 高山 王美艳 雷涛[1] LIU Jia-wei;SHI Xiu-mei;YU Feng-bo;GAO-Shan;WANG Mei-yan;LEI-Tao(College of Pharmacy, Mudanjang Medical College, Mudanjiang 157011, China)

机构地区：[1]牡丹江医学院药学院,黑龙江牡丹江157011

出　　处：《实验室科学》2017年第6期29-30,34,共3页Laboratory Science

基　　金：黑龙江省自然科学基金项目"黑人参糖蛋白的提取;分离及抗肿瘤研究"(项目编号:H2016090);牡丹江医学院科学技术研究项目"黑参粗多糖抑制SMMC-7721;SCG-7901肿瘤细胞增殖的研究"(项目编号:ZS201513)

摘　　要：目的:研究黒参粗多糖对肿瘤细胞SCG-7901和SMMC-7721的增殖抑制作用。方法:采用MTT法(噻唑蓝比色法),以人参粗多糖为阳性对照药物,在490nm波长,检测不同剂量的黒参粗多糖提取液,对肿瘤细胞抑制作用的OD值,考察提取液对肿瘤细胞的24h抑制作用。结果:黒参粗多糖对SGC-7901和SMMC-7721的IC50(半抑制浓度)分别为014475mg/mL和015789mg/mL,黒参粗多糖对肿瘤细胞SCG-7901和SMMC-7721的增殖具有明显的抑制作用,且具有量效关系。Objective： Study on cell SMMC-7721 and SCG-7901 inhibition rates of grude polysaccharides derived from black Ginseng. Methods： The inhibitiory effect on the cells treated by grude polysaccharides of black Ginseng. With different concentration was evaluated by methyl thiazolyl tetrazolium（MTT） assay for 24 hours respectively. The cells treated by grude polysaccharides of Ginseng were used as positive control group, then detect the absorbance at 490 nm by ultraviolet spectrophotometer.Results： Experimentations show the better inhibition rates on cell SMMC-7721 and SCG-7901. SGC-7901： IC_（50）= 0 14475 mg/mL; SMMC-7721： IC_（50）= 0 15789 mg/mL. The growth inhibition effect was time and dosage dependent.

关 键 词：人参 黒参 粗多糖 SGC-7901 SMMC-7721 

分 类 号：R733[医药卫生—肿瘤]