无机碳供应与光照条件对坛紫菜光合功能的影响  

Effects of inorganic carbon supplies and light on photosynthetic functions of Pyropia haitanensis

作　　者：姜恒 邹定辉 娄文勇[2] 

机构地区：[1]华南理工大学环境与能源学院,广州510006 [2]华南理工大学食品科学与工程学院,广州510006

出　　处：《应用生态学报》2018年第2期515-521,共7页Chinese Journal of Applied Ecology

基　　金：广东省科技计划项目(2015A020216004);国家自然科学基金项目(41276148)资助~~

摘　　要：海藻群体密度过大常引起海水中CO_2供应和光照强度降低,为探讨这两种环境条件对坛紫菜光合作用的影响,在4种条件下培养坛紫菜,即390μL·L^(-1)(正常空气)+全日光、20μL·L^(-1)(低CO_2供应)+全日光、390μL·L^(-1)+低日光(光照强度为全日光的20%)、20μL·L^(-1)+低日光,测定藻体的碳酸酐酶活性、光合速率,以及不同温度下开放状态光系统Ⅱ最大量子产量(F_ v'/F _m').结果表明:低CO_2供应和低日光下生长的坛紫菜具有较高的碳酸酐酶活性,并且低日光能够提高海藻最大碳饱和光合放氧速率(V _(max)).在低日光下,生长在低CO_2海水中的坛紫菜V _(max)显著低于正常CO_2海水中生长的海藻V _(max);在全日光下,低CO_2下生长的海藻V _(max)却高于正常CO_2下生长的海藻V _(max).生长在低CO_2和低日光条件下的海藻在低温(10℃)和高温(30℃)下,藻体的F_ v'/F _m'变化不明显;而生长在390μL·L^(-1)+全日光环境中的海藻在高温环境下200 min后,藻体的F_ v'/F _m'相比40 min时的F_ v'/F _m'降低76.4%.低CO_2和低日光提高坛紫菜光合无机碳利用能力以及适应短期温度变化的能力,而低CO_2对海藻光合速率的影响与海藻所处光照环境有关.The increasing stocking density of algal often results in lowered carbon and light levels. In this study, Pyropia haitanensis were cultured under four different conditions, i.e., 390 μL . L-1 （ambient carbon supply） ＋ ambient s sunlight, 390 μL . L-1＋ decreased sunlight t, 20 μL . L-1 （decreased carbon supply） ＋ ambient （20% of ambient sunlight）, 20 μL . L-1＋ decreased sunlight. The activity of carbonic anhydrase （CA）, photosynthetic rate and quantum efficiency of open photosynthetic system Ⅱ （Fv＇/Fm＇） of the algae under different temperatures were measured to investigate the effects of decreased carbon supply and lowered light on photosynthetic functions of P. haitanensis. The results showed the CA activities of the algae at decreased carbon supply and lowered sunlight was increased. The maximum Ci-saturated photosynthetic rates （Vmax ） were elevated under the low sunlight compared with that under the ambient sunlight. The Vmax of the algae at low carbon seawater was lower than that at ambient carbon seawater under lowered sunlight. Under ambient sunlight, the Vmax of the decreased carbon-grown algae was higher than that of ambient carbon-grown algae. The Fv ＇/Fm＇ values of the algae grown at lowered carbon supply and decreased sunlight conditions showed no variation at low temperature （ 10 ℃ ） and high temperature （30 ℃ ）, whereas it was decreased by about 76.4% when cultured at 390 μL . L-1＋ ambient sunlight at 200 min than that at 40 min under 30 ℃. These results suggested that decreased carbon supply and lowered sunlight intensity would improve in the capacity of HCO3- utilization and resistance to short-term temperature change in P. haitaneisis. The effects of low carbon on photosynthetic rate of the algae were dependent on light context.

关 键 词：低CO2 低光照 坛紫菜 碳酸酐酶 光合速率 叶绿素荧光 

分 类 号：S917.3[农业科学—水产科学]