Schizochytrium sp.S31生产DHA底物流加策略及呼吸特性分析  

Substrate flow strategy for producing DHA by Schizochytrium sp. S31 and respiration characteristics analysis

作　　者：孙佼文 丁健[1] 贾禄强 陈珊珊[1] 槐强强 史仲平[1] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《中国油脂》2018年第2期110-114,共5页China Oils and Fats

基　　金：江南大学自主科研计划项目(JUSRP11536)

摘　　要：考虑到培养液内的碳源和氮源质量浓度及其比例关系对细胞生长以及油脂积累的特性有明显的影响,采用分批补料、间歇流加方式考察底物流加策略对Schizochytrium sp.S31生产DHA的影响,同时对发酵尾气CO_2释放与DHA含量的相关性进行分析。结果表明:通过分批培养的方式确定最佳的初始碳氮比为7∶1,此条件下最终的DHA含量达到2.66 g/L;最佳的底物流加策略为培养80 h内间歇流加酵母提取物使培养液中酵母提取物质量浓度达5 g/L,同时将葡萄糖质量浓度控制在30 g/L的恒定水平,DHA含量最终提高至16.75 g/L;分析呼吸特性发现,氮源耗尽后平均CO_2释放速率与DHA的积累量之间存在正相关性。Considering that the mass concentrations of carbon and nitrogen in culture medium and their proportion had a significant effect on cell growth and oil accumulation, the effect of substrate flow strategy on production of DHA by Schizochytrium sp. S31 was studied using batch culture and intermittent flow method, and the correlation between CO2 release in fermentation tail gas and the accumulation of DHA was analyzed. The results showed that the optimal initial C/N ratio was 7：1 by batch culture, and the fi- nal DHA content was 2.66 g/L under this condition. The optimal substrate flow strategy was that the yeast extract was intermittently fed in the first 80 h to make sure the mass concentration of yeast extract in culture medium reaching 5 g/L, and the glucose mass concentration was controlled at a constant level of 30 g/L, and the DHA content finally increased to 16.75 g/L. The respiration characteristics analysis re- sults showed that there was a positive correlation between the average CO2 release rate and the accumula- tion of DHA after depletion of nitrogen.

关 键 词：裂殖壶菌 底物流加 DHA CO2释放速率 

分 类 号：TS222[轻工技术与工程—粮食、油脂及植物蛋白工程] Q815[轻工技术与工程—食品科学与工程]