响应面优化超声波辅助复合酶法提取杏鲍菇黄酮  

Optimization of Ultrasonic Assisted Compound Enzyme Extraction of Flavonoids from Pleurotus eryngii by Response Surface

作　　者：王广慧[1] 李士慧 梁婷[1] 寇送波 信悦 张诺 WANG Guanghui;LI Shihui;LIANG Ting;KOU Songbo;XIN Yue;ZHANG Nuo(College of Food and Pharmaceutical Engineering, Suihua University, Suihua 152061, China)

机构地区：[1]绥化学院食品与制药工程学院,黑龙江绥化152061

出　　处：《东北农业科学》2018年第1期45-50,共6页Journal of Northeast Agricultural Sciences

基　　金：黑龙江省大学生创新创业训练计划项目(201610236011);绥化学院科研创新团队项目(SC2014002)

摘　　要：研究了利用超声波辅助复合酶法提取杏鲍菇黄酮的最优条件。在单因素试验的基础上,固定其他影响因素,选择料液比、超声时间和酶解pH进行三因素三水平的Box-Behnken试验,利用Design-expert 8.0.6软件进行响应面分析。结果表明,在料液比为1∶50(g∶m L),超声时间为6 min,酶解pH为5.4条件下获得的黄酮提取率最高,可达3.352%。The optimum conditions of extraction of flavonoids from Pleurotus eryngii were studied by ultrasonic assisted complex enzyme method. Box-Behnken test of three factors and three levels was performed based on single factor test. Other factors fixed, the ratio of solid to liquid, ultrasonic time and enzymolysis pH were selected. Response surface analysis was performed by Design-expert 8.0.6. The results showed that the highest extraction rate was 3.352%, the ratio of material to liquid was 1：50 （g/mL）, the ultrasonic time was 6 rain and the pH value was 5.4.

关 键 词：响应面 超声波 复合酶 杏鲍菇 黄酮 

分 类 号：S646[农业科学—蔬菜学]