高效液相色谱-串联质谱法测定保健食品中牛磺酸  

HPLC-MS/MS Determination of Taurine in Health-Care Food

作　　者：康明芹[1] 陈明岩[1] 王岸英[1] 张蕊蕊 徐立明[1] 李荣荣[1] 

机构地区：[1]吉林出入境检验检疫局,长春130062 [2]吉林大学公共卫生学院,长春130021

出　　处：《理化检验（化学分册）》2018年第2期153-156,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家质检总局科技项目(2012IK162;2013IK163)

摘　　要：采用高效液相色谱-串联质谱法测定保健食品中牛磺酸的含量。称取保健食品样品适量(精确至0.001g),制成溶液并加水稀释至一定体积,液体制剂可直接用水稀释。所得样品溶液分别用于分析。以BEH Amide色谱柱为分离柱,以不同体积比的乙腈(A)和水(B)的混合液为流动相进行梯度洗脱,采用电喷雾负离子源多反应监测模式检测。牛磺酸的质量浓度在0.01~2.0mg·L^(-1)内与其对应的峰面积呈线性关系,测定下限(10S/N)为1.0mg·kg^(-1)。方法用于保健食品样品的分析,加标回收率为94.0%~102%,测定值的相对标准偏差(n=6)为0.67%~3.9%。HPLC-MS/MS was applied to the determination of taurine in health-care food. An appropriate amount of the health-care food sample was weighed （to the nearest 0. 001 g） and dissolved. The solution was diluted to a definite volume with water. Liquid sample could diluted with water directly. The sample solution obtained was used for analysis. BEH Amide chromatographic column was used as stationary phase, and mixtures of acetonitrile （A） and water （B） in different ratios were used as mobile phase in gradient elution. Mode of ESI- and multireaction monitoring mode were adopted in MS/MS. Linear relationship between values of peak area and mass concentration of taurine was kept in the range of 0. 01- 2. 0 mg · L-1, with lower limit of determination （10S/N） of 1. 0 mg· kg -1. The proposed method was applied to the analysis of health food samples, giving values of recovery and RSD＇s （n=6） in the ranges of 94. 0% --102% and 0. 67% --3.9% respectively.

关 键 词：高效液相色谱-串联质谱法 牛磺酸 保健食品 

分 类 号：O657.63[理学—分析化学]