Development of BCPDA-Eu^3＋-BAS Labeled Hepatitis B Surface Antibodies  

作　　者：TAN Wenjia SUN Zhixia XUAN Lili LI Junling XIE Wenbing HE Chengyan PAN Lihua 

机构地区：[1]China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China [2]Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China

出　　处：《Chemical Research in Chinese Universities》2018年第1期24-27,共4页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China（No. 81572082） and the Natural Science Foundation of Jilin Province, China（Nos.2011713, 20150414015GH, 20150204010YY）.

摘　　要：The effect of the chelating agent 4,7-bis（chlorosulfophenyl）-1,10-phenanthroline-2,9-dicarboxylic acid （BCPDA） labeled hepatitis B surface antibodies（HBsAb） on time-resolved fluoroimmunoassays（TRFIA） was investigated. The labeling detection method was established. The biotin-streptavidin（BAS） system was introduced, and the multi-stage amplification effect of the BAS system was analyzed. It is found that the BCPDA-Eu^3＋-HBsAb-BAS fluorescent marker can emit strong fluorescence. Compared to BCPDA-Eu^3＋-HBsAb, BCPDA-Eu^3＋-HBsAb-BAS demonstrates an enhanced fluorescence intensity by over 10 times. This study provides a guidance for the next generation non-radio immunoassays in clinical diagnosis,The effect of the chelating agent 4,7-bis（chlorosulfophenyl）-1,10-phenanthroline-2,9-dicarboxylic acid （BCPDA） labeled hepatitis B surface antibodies（HBsAb） on time-resolved fluoroimmunoassays（TRFIA） was investigated. The labeling detection method was established. The biotin-streptavidin（BAS） system was introduced, and the multi-stage amplification effect of the BAS system was analyzed. It is found that the BCPDA-Eu^3＋-HBsAb-BAS fluorescent marker can emit strong fluorescence. Compared to BCPDA-Eu^3＋-HBsAb, BCPDA-Eu^3＋-HBsAb-BAS demonstrates an enhanced fluorescence intensity by over 10 times. This study provides a guidance for the next generation non-radio immunoassays in clinical diagnosis,

关 键 词：Time-resolved fluorescence immunoassay BIOTIN STREPTAVIDIN 

分 类 号：S858.315.3[农业科学—临床兽医学] O484[农业科学—兽医学]