液相色谱/元素分析仪-同位素质谱法鉴定蜂蜜掺假的方法改进  

Optimization of identification of honey adulteration by liquid chromatography/elementary analysis-isotope ratio mass spectrometry

作　　者：季美泉 林宏[1] 丁涛[1] 王艳[1] 桂茜雯[1] 刘芸[1] 张建[1] 费晓庆[1] 谭梦茹 赵梦婷 

机构地区：[1]江苏出入境检验检疫局食品实验室,南京210019 [2]无锡市药品安全检验检测中心,无锡214000 [3]东南大学成贤学院,南京210088

出　　处：《食品安全质量检测学报》2018年第3期498-505,共8页Journal of Food Safety and Quality

基　　金：江苏出入境检验检疫局科技计划项目(2017KJ18)~~

摘　　要：目的改进液相色谱/元素分析仪-同位素质谱法鉴别蜂蜜掺假的方法。方法对现有欧盟标准方法优化液相色谱条件,结合元素分析仪-同位素质谱法,将二糖分离为麦芽糖、蔗糖,提出一个新的参数—麦芽糖、蔗糖δ^(13)C值之差δ^(13)C_(M-S)。结果根据本研究检测113个国内外不同来源纯正蜂蜜样本的数据,提出纯正蜂蜜δ^(13)C值新要求:蜂蜜蛋白质与蜂蜜同位素差值δ^(13)C_(P-H)大于-0.97‰;果糖、葡萄糖δ^(13)C值之差δ^(13)C_(F-G)在-0.60‰至0.56‰范围内;麦芽糖、蔗糖δ^(13)C值之差δ^(13)C_(M-S)在-0.73‰至0.98‰范围内;各个组分δ^(13)C最大差值δ^(13)C_(max)小于2.05‰;根据上述4个参数来确认蜂蜜是否掺假。在日常检测和市场销售的160个样品中,原方法阳性检出率为16.2%,而新方法阳性检出率达21.9%。结论本研究提升了蜂蜜掺假检测能力,此方法的建立更好、更精确打击掺假的同时,也维护消费者权益。Objective To optimize the method for identification of honey adulteration by liquid chromatography/elementary analysis-isotope ratio mass spectrometry.Methods According to EU standard methods,the liquid chromatography conditions were optimized and element analyzer-isotopic mass spectrometry were combined.Disaccharide was separated into maltose and sucrose.A new parameter,δ^（13）C difference between maltose and sucrose（δ^（13）C_（M-S））,was presented.Results Based on the isotopic data of 113 authentic honeys from different sources at home and abroad,theδ^（13）C value limits for the authentic honeys were proposed：δ^（13）C difference between honey and protein（δ^（13）C_（P-H））was more than-0.97‰,δ^（13）C difference between fructose and glucose（δ^（13）C_（F-G））was from-0.60‰to 0.56‰,δ^（13）C_（M-S） was from-0.73‰to 0.98‰and the maximum difference between allδ^（13）C values should be less than 2.05‰（δ^（13）C_（max）2.05‰）.Under the pure honey criteria,the positive percentage by the new method was21.9%for 160 commercial samples,while the positive percentage by the old method was only 16.2%.Conclusion This study improves the detection ability of honey adulteration,which is better and more accurate to combat adulteration,while maintaining the rights and interests of consumers.

关 键 词：蜂蜜掺假 同位素质谱 液相色谱 

分 类 号：O657.63[理学—分析化学] S896.1[理学—化学]