质谱特征离子定性分析海洋鱼油中的脂肪酸组成  

Qualitative Analysis of Fatty Acid Composition of Marine Fish Oil by Characteristic Ions

作　　者：楼乔明[1,3] 李来好[2] 陈胜军[2] 杨文鸽[1] 张进杰[1] 薛长湖[3] 

机构地区：[1]宁波大学海洋学院,浙江宁波315211 [2]中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,广州510300 [3]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《中国食品学报》2017年第12期193-200,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31201283);“水产”浙江省重中之重学科开放基金项目(xkzsc1531);农业部水产品加工重点实验室开放基金项目(nybjg201208)

摘　　要：研究质谱特征离子对海洋鱼油脂肪酸的快速定性分析。首先,根据脂肪酸甲酯的断裂规律,基峰离子可快速确定脂肪酸的不饱和度,如基峰离子m/z 74为饱和脂肪酸,m/z 55为单烯脂肪酸,m/z 67为二烯脂肪酸,m/z 79为三烯及以上多不饱和脂肪酸;其次,分子离子M+及其特征性碎片丢失而形成的特征离子如[M-29]^+、[M-31]^+、[M-32]^+、[M-43]^+和[M-74]^+等,可有效用于脂肪酸甲酯分子量和脂肪酸碳链长度的分析;而ω离子和α离子可准确对三烯及以上多不饱和脂肪酸中的双键位置进行分析鉴定。通过质谱特征离子的综合分析,从海洋鱼油胶囊中共分析鉴定出24种脂肪酸,以C_(14:0)、C_(16:0)、C_(18:1n-9)、C_(20:5n-3)和C_(22:6n-3)为主,表明质谱特征离子能快速准确用于海洋鱼油脂肪酸的定性分析。同时,海洋鱼油中C_(20:5n-3)和C_(22:6n-3)的总含量高达29.13%,表明其具有很高的营养价值和保健功能。Qualitative analysis of fatty acid composition of marine fish oil was studied by characteristic ions in this paper. Firstly, the degrees of unsaturation of fatty acids were determined quickly by base-peak ions, m/z 74 as saturated fatty acids, m/z 55 as monoene fatty acids, m/z 67 as diene fatty acids and m/z 79 as polyunsaturated fatty acids with three double-bonds or more, according to mass spectrometric fragmentation rules. Secondly, molecular weights and carbon chain lengths could be effectively identified by molecular ion（M＋） and characteristics ions with fragment losses, such as[M-29]＋, [M-31]＋, [M-32]＋, [M-43]＋ and [M-74]＋. Moreover, the double-bond positions of polyunsaturated fatty acids with three double-bonds or more were fast determined by ω ions and α ions. Twenty-four kinds of fatty acids were identified using characteristic ions from fish oil capsules, and the main fatty acids were C14：0, C16：0, C18：1 n-9, C20：5 n-3 and C22：6 n-3, showing that characteristic ions could be applied to qualitative analysis of fatty acid composition of marine fish oil rapidly and accurately. Meanwhile, the total content of C20：5 n-3 and C22：6 n-3 was as high as 29.13%, showing that the marine fish oil had high nutritional values and health functions.

关 键 词：海洋鱼油 脂肪酸 特征离子 定性分析 

分 类 号：O657.63[理学—分析化学] TS225.24[理学—化学]