一起由多种血清型副溶血性弧菌引起的食物中毒的实验室检测分析  

Laboratory detection of several species of Vibrio parahaemolyticus in a food poisoning

作　　者：杨洋[1] 黎明[1] 李涛[1] 苗艳芳[1] 孔喜梅 

机构地区：[1]成都市疾病预防控制中心,四川成都610051

出　　处：《中国卫生检验杂志》2018年第2期176-178,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的研究引起本次食物中毒的副溶血性弧菌血清型和分子分型的特征。方法采集患者肛拭子、剩余食物和水样样本进行致病菌检测,对分离的可疑致病菌进行鉴定、PCR毒力基因检测和脉冲场凝胶电泳(PFGE)分子分型。结果从27份样本中分离出16株副溶血性弧菌,分属8个血清型;经PFGE分型,16株菌共产生11种带型,相似度为51.45%;12株菌携带tdh基因,所有菌株均不带有trh基因。结论此次食物中毒由多型别的副溶血性弧菌混合感染引起。建议在处理由副溶血性弧菌引起的食物中毒的过程中,每一个样本均需挑取多个可疑菌落,留待后续的检测。Objective To study the serotype and molecular type of a restaurant food poisoning caused by Vibrio parahaemolyticus. Methods Patients＇ anal swabs,residual food and water samples were collected for pathogen detection,and the isolated suspected pathogens were conducted for identification,PCR virulence gene detection and pulsed-field gel electrophoresis（ PFGE） molecular typing. Results A total of 16 strains of Vibrio parahaemolyticus were isolated from patients＇ samples,which belonged to 8 serotypes and produced 11 patterns by PFGE. The similarity coefficient was 51. 45%. 12 strains carried tdh gene,no strain carried trh gene. Conclusion The food poisoning is caused by multiple types of mixed infection of Vibrio parahaemolyticus. It is recommended that in dealing with food poisoning caused by Vibrio parahaemolyticus,multiple suspicious colonies should be picked for each sample for subsequent testing.

关 键 词：副溶血性弧菌 实验室检测 脉冲场凝胶电泳 

分 类 号：R155.3[医药卫生—营养与食品卫生学]