松乳菇多糖提取工艺优化及抗氧化活性评价  

Optimization of extraction technology of polysaccharide and antioxidant activity determination from Lactarius deliciosuss

作　　者：廖国会 龙家寰 秦立新 段婷婷 

机构地区：[1]贵州省植物保护研究所,贵州贵阳550006

出　　处：《食品工业科技》2018年第4期123-130,共8页Science and Technology of Food Industry

基　　金：贵州省服务企业行动计划(20154012);贵州省农业科学院重点项目(06005)

摘　　要：为了探索松乳菇菌丝体多糖的最佳提取工艺,并对其多糖进行体外抗氧化活性初步研究。采用超声波辅助浸提的方法,以温度、时间、料液比和次数进行单因素实验;在此基础之上,利用Box-Benhnken方法进行四因素三水平实验设计,以多糖得率为响应值,进行响应面分析;通过测定多糖清除DPPH自由基、OH自由基和O-2自由基的能力来评价其抗氧化活性,并与维生素C进行对比。结果表明,松乳菇多糖最佳提取工艺条件为:提取温度92.8℃、提取时间1.6 h、料液比1∶28(g∶m L)和提取次数3次,此条件下松乳菇多糖得率预测值为10.60%,实测值为10.41%,与预测值相对误差为1.79%,说明优化工艺可行。松乳菇多糖对DPPH自由基、OH自由基和O-2自由基都具有一定的清除能力,其IC_(50)值分别为0.855,1.147,1.126 mg/m L;但与维生素C比较,其抗氧化活性较弱。热水浸提法提取松乳菇多糖高效、简单、低成本,可用作松乳菇多糖的提取工艺;松乳菇多糖具有明显的体外抗氧化活性。To optimize the extraction technology of polysaccharides from Lactarius deliciosus,and the antioxidant activities of the polysaccharides.The single-factor experiment was carried out by using ultrasonic-assisted extraction method with temperature,time,solid-liquid ratio and times.On the basis of single-factor experiments,a four-factor,three-level Box-Behnken central composite design was utilized for mathematical modeling for polysaccharide yield,followed by responses surface analysis of the relationship between the function and the four variables. In vitro antioxidation activity of the L. deliciosus polysaccharides was evaluated by DPPH,OH and O-2free radicals scavenging method comparing with ascorbic acid. The results showed that the optimal extraction technology of polysaccharides from L.deliciosus were as follows： extraction temperature of 92.8 ℃,extracting time of 1.6 h,solid-liquid ratio of 1∶ 28（g∶ m L）,and extraction times of 3.Under these conditions,the model-predicted and experimentally measured values of L.deliciosus polysaccharide yield were 10.60% and 10.41%,respectively,revealing a relative error of only 1.79% between them.Thus,the conditions were optimal for polysaccharide extraction.The polysaccharides from L.deliciosus had a capacity to scavenge DPPH free radical,hydroxyl free radical,superoxide anion,and the IC_（50） was 0.855,1.147 and 1.126 mg/m L,respectively. Antioxidant activity of L. deliciosus polysaccharides was weaker than those of vitamin C. The extraction technology was efficient,simple low cost and applicable for the extraction of polysaccharides form L.deliciosus and L.deliciosus polysaccharides has significant in vitro antioxidation.

关 键 词：松乳菇 响应面法 多糖 抗氧化活性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]