响应面优化串叶松香草总黄酮的提取工艺及其抗氧化活性研究  

Optimization of extraction process and antioxidant activity of total flavonoids from cup plant by response surface method

作　　者：邴鑫 尚红梅[1] 娄玉杰[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《食品工业科技》2018年第4期137-142,149,共7页Science and Technology of Food Industry

基　　金：国家自然科学基金青年科学基金项目(31601972)

摘　　要：通过响应面法对串叶松香草中的总黄酮提取工艺进行优化。根据Box-Behnken中心组合原理,在醇提法和单因素实验法基础上将显著效应的四个单因素:液料比、提取时间、提取温度及提取液乙醇浓度进行四因素三水平的响应面设计实验,以获得串叶松香草总黄酮提取工艺参数,并测定串叶松香草中总黄酮的抗氧化活性。最优实验工艺条件为:液料比31∶1 m L/g、提取时间129 min、提取温度38℃、乙醇浓度42%。在此条件下获得的总黄酮提取量高达(38.93±0.012)mg/g,与理论值相差0.04%。1 mg/m L的串叶松香草总黄酮(0.51 mg)对DPPH自由基的清除率为73.06%,0.6 mg/m L的串叶松香草总黄酮ABTS+自由基的清除率为100%,6 mg/m L的串叶松香草总黄酮还原力为0.188,说明串叶松香草总黄酮具有较强的自由基清除活性,具有一定还原力。The extraction of total flavonoids from cup plant was optimized through response surface methodology. According to the Box-Behnken response surface method and on the basis of ethanol extraction and single-factor test,four single factor having significantly effects were selected： liquid-to-solid ratio,extraction time,extraction temperature and alcohol concentration to finish response surface design experiment with four factors and three levels. The extraction yield of total flavonoids as responses was optimized by response surface analysis to obtain the extraction parameters.The antioxidant activity of total flavonoids from cup plant was determined.The optimal extraction conditions of total falvonoids were liquid-to-solidratio of 31∶ 1（m L/g）,extraction time of 129 min,extraction temperature at 38 ℃ for 42% with alcohol concentration.The extraction yield of total flavonoids obtained under these conditions was as high as（38.93 ± 0.012） mg/g which differed with the theoretical value of 0.04%.The extracting of 1 mg/m L total flavonoids（0.51 mg） had strong scavenging capacity on DPPH radicals rate that was 73.06%.Scavenging activity of total flavonoids from cup plant by 0.6 mg/m L against ABTS＋free radicals was 100%.Ferric reducing power from total flavonoids of cup plant of by 6 mg/m L was 0.188. Response surface methodology was applicable for the extraction optimization of total flavonoids from cup plant.The total flavonoids from cup plant had strong radical scavenging capacity and a certain ferric reducing power.

关 键 词：串叶松香草 总黄酮 提取 响应面 抗氧化 

分 类 号：TS201.1[轻工技术与工程—食品科学]