制备黑曲霉孢子粉干燥方法及其保护剂研究  

Optimization of drying methods and dried protectant formulation of spore powder of Aspergillus niger

作　　者：黄娟[1] 张琳 张岚 黎恒 王德培[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《食品科技》2018年第2期21-25,共5页Food Science and Technology

基　　金：2016年山东省重点研发计划项目(2016GRC3201)

摘　　要：在35℃烘干工艺条件下,采用单因素试验和正交试验设计,对黑曲霉孢子粉干燥保护剂配方进行筛选和优化。再通过不同真空度干燥法及干燥时间之间的相互比较,得出最佳孢子粉干燥工艺。结果表明,最佳保护剂配方为海藻糖7.5 g/100 mL、蛋白胨2 g/100 mL及甘油3 g/100 mL;最佳孢子粉干燥工艺为35℃、0.8×10~5 Pa真空度下干燥16 h,孢子存活率为95.81%。干燥在4℃和20℃下贮存孢子粉并进行为期6个月的孢子存活率测定,结果显示,在4℃下贮存6个月后的孢子存活率仍达86.79%,而20℃下的存活率降至53.15%。证明孢子粉在较低温(4℃)下贮存效果更好。Dried protectant formulation of spore powder of Aspergillus niger was screened and optimized by single factor test and orthogonal design at 35 ℃ under drying process conditions. The best drying process of spore powder was obtained by comparing the drying methods with different vacuum degrees and drying time. The results showed that the optimal dried protectors formulation（per 100 m L of dried protectant） consisted of 7.5 g of trehalose, 2.0 g of peptone, and 3.0 g of glycerol; the optimal drying process were temperature of 35 ℃ the vacuum of 0.8×10~5 Pa and 16 h. Spore powder of Aspergillus niger were stoerd at 4 ℃ and 20 ℃, their viability were examined at 1 month intervals. The survival rate of spores remained 86.79% at 4 ℃ months after storage for 6 months, while the survival rate was reduced to 53.15% at 20 ℃. It was proved that spore powder stored at lower temperature（4 ℃）was better.

关 键 词：黑曲霉 孢子粉 保护剂 干燥工艺 存活率 

分 类 号：TS201.3[轻工技术与工程—食品科学]