微卫星DNA标记对BABL/c突变卷毛小鼠遗传特性的分析  

Analysis of genetic characteristics of BABL/c mutant curly mice by microsatellite DNA markers

作　　者：李晓娟[1] 孙兆增[2] 冯帆 姜棋予 孙慧伟 李润 柴燕涛[1] 侯俊[1] 李瑞生[1] 

机构地区：[1]解放军第302医院临床研究管理中心,北京100039 [2]军事医学科学院实验动物中心,北京100071

出　　处：《中国比较医学杂志》2018年第2期80-84,共5页Chinese Journal of Comparative Medicine

基　　金：军队实验动物专项基金面上项目(SYDW(2016)-005)

摘　　要：目的利用微卫星DNA标记技术对BALB/c突变卷毛小鼠与正常BALB/c小鼠进行遗传检测,旨在分析BALB/c突变卷毛小鼠与正常小鼠之间存在的遗传差异。方法选取38个微卫星DNA位点结合荧光PCR技术和STR扫描基因型来检测BALB/c突变卷毛小鼠、无毛小鼠与正常小鼠三个群体的遗传变异情况。结果 38个微卫星位点在BALB/c突变卷毛小鼠与正常小鼠之间有27个微卫星位点相同,有11个位点存在差异,其突变率为28.9%(11/38);BABL/c突变无毛小鼠与正常小鼠之间有30个位点完全相同,8个位点存在差异,其突变率为21.1%(8/38);而BABL/c突变卷毛小鼠与无毛小鼠间也有12个位点存在差异。结论 BALB/c突变卷毛小鼠的突变率较高,且明显高于无毛小鼠,证明了卷毛小鼠突变与无毛小鼠突变是两个完全不同的突变系,为今后研究和开发应用BALB/c突变卷毛小鼠提供可靠的理论数据。Objective BALB/c mutant curly mice and normal BALB/c mice were genetically detected by microsatellite DNA marker analysis to detect the differential microsatellite loci between BALB/c mutant curly mice and normal mice. Methods 38 microsatellite DNA loci were selected and their variation in the BALB/c mutant curly mice,BALB/c mutant hairless mice and normal BALB/c mice were detected by multiplex fluorescence PCR and STR scanning genotyping. Results There were 27 the same microsatellite loci between the 38 microsatellite loci in BALB/c mutant curly mice and normal mice,and there were 11 differential loci,with a mutation rate of 28. 9%（ 11/38）. There were 30 the same sites between BABL/c mutant hairless mice and normal mice,and there were 8 different loci,with a mutation rate of21. 1%（ 8/38）. There were also 12 differential loci between BABL/c mutant curly mice and hairless mice. Conclusions BALB/c mutant curly mice have a higher mutation rate and are significantly higher than those of hairless mice,demonstrating that the mutations in curly mice and hairless mice are two completely different mutations. These results provide reliable theoretical data for the future study and development of BALB/c mutant curly mice.

关 键 词：BALB/c突变卷毛小鼠 STR 荧光PCR 

分 类 号：R-33[医药卫生]