MicroRNA Profiling of Transgenic Mice with Myocardial Overexpression of Nucleolin  

作　　者：Qing-Lan Lyu Bi-Mei Jiang Bin Zhou Li Sun Zhong-Yi Tong Yuan-Bin Li Yu-Ting Tang Hui Sun Mei-Dong Liu Xian-Zhong Xiao 

机构地区：[1]Department of Pathophysiology, Xiangya School of Basic Medical Sciences, Central South University, Changsha, Hunan 410078, China [2]Department of Rheumatology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China [3]Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410000, China

出　　处：《Chinese Medical Journal》2018年第3期339-346,共8页中华医学杂志（英文版）

摘　　要：Background：Nucleolin （NCL） is the most abundant RNA-binding protein in the cell nucleolus and plays an important role in chromatin stability,ribosome assembly,ribosomal RNA maturation,ribosomal DNA transcription,nucleocytoplasmic transport,and regulation of RNA stability and translation efficiency.In addition to its anti-apoptotic properties,the underlying mechanisms associated with NCL-related roles in different cellular processes remain unclear.In this study,the effect of NCL on microRNA （miRNA） expression was evaluated by generating transgenic mice with myocardial overexpression of NCL and by analyzing microarrays of mature and precursor miRNAs from mice.Methods：Using microinjection ofalpha-MyHc clone 26-NCL plasmids,we generated transgenic mice with myocardial overexpression of NCL firstly,and then mature and precursor miRNAs expression profiles were analyzed in NCL transgenic mice （n =3） and wild-type （WT） mice （n =3） by miRNA microarrays.Statistical Package for the Social Sciences version 16.0 software （SPSS,Inc.,Chicago,IL,USA） was used to perform Student＇s t-test,and statistical significance was determined at P 〈 0.05.Results：Several miRNAs were found to be differentially expressed,of which 11 were upregulated and 4 were downregulated in transgenic mice with myocardial overexpression of NCL compared to those in WT mice.Several differentially expressed miRNAs were subsequently confirmed and quantified by real-time quantitative reverse transcription-polymerase chain reaction.Bioinformatics analysis was used for the prediction of miRNA targets.Furthermore,in vitro experiments showed that NCL regulated miR-21 expression following hydrogen peroxide preconditioning.Conclusions：Myocardial-protection mechanisms exerted by NCL might be mediated by the miRNAs identified in this study.Background：Nucleolin （NCL） is the most abundant RNA-binding protein in the cell nucleolus and plays an important role in chromatin stability,ribosome assembly,ribosomal RNA maturation,ribosomal DNA transcription,nucleocytoplasmic transport,and regulation of RNA stability and translation efficiency.In addition to its anti-apoptotic properties,the underlying mechanisms associated with NCL-related roles in different cellular processes remain unclear.In this study,the effect of NCL on microRNA （miRNA） expression was evaluated by generating transgenic mice with myocardial overexpression of NCL and by analyzing microarrays of mature and precursor miRNAs from mice.Methods：Using microinjection ofalpha-MyHc clone 26-NCL plasmids,we generated transgenic mice with myocardial overexpression of NCL firstly,and then mature and precursor miRNAs expression profiles were analyzed in NCL transgenic mice （n =3） and wild-type （WT） mice （n =3） by miRNA microarrays.Statistical Package for the Social Sciences version 16.0 software （SPSS,Inc.,Chicago,IL,USA） was used to perform Student＇s t-test,and statistical significance was determined at P 〈 0.05.Results：Several miRNAs were found to be differentially expressed,of which 11 were upregulated and 4 were downregulated in transgenic mice with myocardial overexpression of NCL compared to those in WT mice.Several differentially expressed miRNAs were subsequently confirmed and quantified by real-time quantitative reverse transcription-polymerase chain reaction.Bioinformatics analysis was used for the prediction of miRNA targets.Furthermore,in vitro experiments showed that NCL regulated miR-21 expression following hydrogen peroxide preconditioning.Conclusions：Myocardial-protection mechanisms exerted by NCL might be mediated by the miRNAs identified in this study.

关 键 词：MicroRNA microarray MYOCARDIUM NUCLEOLIN Transgenic mice 

分 类 号：Q522[生物学—生物化学] Q78