紫色马铃薯花色苷改善HepG2细胞胰岛素抵抗模型糖代谢的机制  

The mechanism of purple potato anthocyanins improve the insulin resistance model of HepG2 cells

作　　者：徐华容[1,2] 李丽琪[1,2] 刘阳[1] 姚少成 沈蔓芸 操青 唐志玮 阮金兰[1,2,3] 

机构地区：[1]武昌理工学院生命科学学院,武汉430223 [2]武汉工程大学化工与制药学院,武汉430073 [3]华中科技大学同济医学院,武汉430030

出　　处：《食品工业科技》2018年第5期319-324,共6页Science and Technology of Food Industry

基　　金：湖北省协同创新中心生物多肽糖尿病药物(鄂教科函[2012]56号);湖北省生物多肽糖尿病药物工程技术研究中心(鄂科技通[2014]72号)基金

摘　　要：目的:观察紫色马铃薯花色苷对HepG2细胞胰岛素抵抗模型糖代谢的作用并对其机制进行初探。方法:以高浓度葡萄糖培养基为诱导因素诱导HepG2细胞建立胰岛素抵抗细胞模型,通过葡萄糖氧化酶法、四唑盐比色实验(MTT)和蛋白质印迹法(Western blot)检测紫色马铃薯花色苷对胰岛素抵抗细胞模型的葡萄糖消耗量、细胞存活率和胰岛素信号通路的蛋白表达的影响。结果:与空白对照组相比,当诱导培养基中葡萄糖浓度为50 mmol/L时吸光度的差值最大,所以选50 mmol/L的葡萄糖培养基来建立胰岛素抵抗细胞模型;花色苷的处理胰岛素抵抗细胞的浓度为40~100μg/mL时,葡萄糖消耗量可高达19.55 mmol/L显著(p<0.05)高于模型对照组;花色苷浓度为40~100μg/mL时细胞存活率基本上可以保持在80%,细胞毒性较小;花色苷可以调节因高浓度葡萄糖诱导而导致的IR、IRS-1、IRS-2水平下降的情况,降低p-IRS-1的表达水平,还可以阻止GLUT-2水平的降低。结论:紫色马铃薯花色苷可以改善HepG2细胞胰岛素抵抗模型的胰岛素信号通路抑制的情况,改善胰岛素抵抗模型的糖代谢。Objective：To observe the effect of purple potato anthocyanin in HepG2 cell insulin resistance model on glucose metabolism and explore its mechanism. Methods： High concentration glucose medium as the induction factor to induce the HepG2 cells establishing insulin resistance cell model.The effects of purple potato anthocyanins on glucose consumption, cell viability and protein expression of insulin signaling pathway were investigated though the glucose oxidase method, tetrazolium colorimetric assay（ MTT） and Western blot.Compared with the blank control group,the difference of absorbance was greatest when the glucose concentration was 50 mmol/L,so 50 mmol/L glucose medium was chosed to establish insulin resistance cell model.When the concentration was 40-100 μg/mL of anthocyanin which was used to treating the insulin resistanc, e cell model, the glucose consumption could be as high as 19.55 mmol/L which was significantly（p 〈 0.05）higher than that of the model control group.When the concentration of anthocyanin was 40- 100 μg/mL, the cell survival rate could be kept at 80% ,with less cytotoxicity.Anthocyanins could regulate the stuations of levels decreasing of IR, IRS-1, IRS-2 due to high concentration of glucose, could reduce the expression level of p- IRS-1 and also prevent the decrease of GLUT- 2 level. Conclusion ： Purple potato anthocyanins could improve the insulin signal pathway inhibition situation of HepG2 cell insulin resistance model and improve the glucose metabolism situation of insulin resistance model.

关 键 词：高浓度葡萄糖 HEPG2细胞 胰岛素抵抗模型 紫色马铃薯花色苷 

分 类 号：TS201.4[轻工技术与工程—食品科学]