联合检测泛素连接酶Cullin1和基质金属蛋白酶MT4-MMP在乳腺癌组织中的表达及临床意义  

Expression and clinical significance of combined detection of Cullin1 and MT4-MMP in breast cancer

作　　者：任毅[1] 李静[1] 张蓬波 钟青[1] 任泽强 

机构地区：[1]江苏省徐州市肿瘤医院乳腺科,221000 [2]徐州医科大学附属医院普外科,221000

出　　处：《中华普通外科学文献（电子版）》2018年第1期19-23,共5页Chinese Archives of General Surgery(Electronic Edition)

基　　金：卫生部科研基金项目(W201302)

摘　　要：目的探究泛素连接酶Cullin1和基质金属蛋白酶MT4-MMP在不同类型乳腺癌组织中表达情况及临床意义。方法 2015年1月至8月,选择徐州市肿瘤医院80例乳腺癌组织及其对应的癌旁正常乳腺组织,应用免疫组织化学法和Western blotting法分别检测其中Cullin1和MT4-MMP的表达情况,并分析其相关性。结果 Cullin1、MT4-MMP蛋白在乳腺癌组织中的表达均高于癌旁正常乳腺组织,差异均有统计学意义(77.5%vs 28.8%,67.5%vs 17.5%,χ~2=38.174、40.921,均P<0.01);在乳腺癌组织中,Cullin1及MT4-MMP蛋白的表达呈正相关(P<0.05)。Cullin1及MT4-MMP在乳腺癌中的蛋白表达在分子分型、腋窝淋巴结是否转移及TNM分期组间差异有统计学意义(P<0.05);在患者年龄、肿瘤大小、肿瘤部位、是否复发转移组间差异无统计学意义。结论 Cullin1及MT4-MMP可能参与了乳腺癌的形成及发展过程,且两者在此过程中有一定协同作用。在分型差、分期晚的乳腺癌中,Cullin1及MT4-MMP更高的表达状态提示其可能成为新的乳腺癌恶性程度指标。Objective To explore the expression and clinical significance of Cullin1 and MT4-MMP in different types of breast cancer tissue.Methods Eighty cases of breast cancer tissues and 80 cases of normal breast tissues adjacent to breast cancer were selected.Immunohistochemistry and Western blotting was used to detect the expression of Cullin1 and MT4-MMP in cancer tissue and normal breast tissue respectively between January 2015 and August 2015 in Xuzhou City Cancer Hospital,and to analyze the correlation between Cullin1 and MT4-MMP.Results The expressions of Cullin1 and MT4-MMP in breast cancer were significantly higher than in adjacent normal breast tissues（77.5% vs 28.8%,67.5% vs 17.5%,χ~2=38.174,40.921,both P〈0.01）.In breast cancer tissues,the expression of Cullin1 and MT4-MMP protein was positively correlated（P〈0.05）.The expression of Cullin1 and MT4-MMP in breast cancer was statistically significant in molecular typing,axillary lymph node metastasis and TNM staging（P〈0.05）,but without significant difference in the age,tumor size,tumor location,recurrence and metastasis.Conclusions Cullin1 and MT4-MMP may be involved in the formation and development of breast cancer and seem to play a certain synergy role in this process.Higher expression level of Cullin1 and MT4-MMP in worse molecular typing and advanced breast cancer may become a new marker of malignant breast cancer.

关 键 词：乳腺肿瘤 SKP CULLIN F-框蛋白质连接酶 基质金属蛋白酶17 

分 类 号：R737.9[医药卫生—肿瘤]