Pristine mesoporous carbon hollow spheres as safe adjuvants induce excellent Th2-biased immune response  

作　　者：Manasi Jambhrunkar Meihua Yu Hongwei Zhang Prasanna Abbaraju Anand Kumar Meka Antonino Cavallaro Yao Lu Neena Mitter Chengzhong Yu 

机构地区：[1]Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia [2]QueenslondAlliance forAgriculture and Food Innovation. The University of Queensland. QLD 4072, Australia

出　　处：《Nano Research》2018年第1期370-382,共13页纳米研究（英文版）

摘　　要：The development of a safe and effective adjuvant that amplifies the immune response to an antigen is important for vaccine delivery. In this study, we developed pristine mesoporous carbon hollow spheres as high-capacity vaccine protein nanocarriers and safe adjuvants for boosting the immune response. Mono-dispersed invaginated mesostructured hollow carbon spheres （IMHCSs） have an average particle size of -200 nm, large pore size of 15 rim, and high pore volume of 2.85 cmB.g-1. IMHCSs exhibited a very high loading capacity （1,040 ~tg-mg-1） towards ovalbumin （OVA, a model antigen）, controlled OVA release behavior, excellent safety profile to normal cells, and high antigen delivery efficacy towards macrophages. In vivo immunization studies in mice demonstrated that OVA-loaded IMHCSs induced a 3-fold higher IgG response compared to a traditional adjuvant QuilA used in veterinary vaccine research. OVA delivered by IMHCSs induced a higher IgG1 concentration than IgG2a, indicating a T-helper 2 （Th2）-polarized response. Interferon-y and interleukin-4 concentration analysis revealed both T-helper 1 （Thl） and Th2 immune responses induced by OVA- loaded IMHCSs. IMHCSs are safer adjuvants than QuilA. Our study revealed that pure IMHCSs without further functionalization can be used as a safe adjuvant for promoting Th2-biased immune responses for vaccine delivery.The development of a safe and effective adjuvant that amplifies the immune response to an antigen is important for vaccine delivery. In this study, we developed pristine mesoporous carbon hollow spheres as high-capacity vaccine protein nanocarriers and safe adjuvants for boosting the immune response. Mono-dispersed invaginated mesostructured hollow carbon spheres （IMHCSs） have an average particle size of -200 nm, large pore size of 15 rim, and high pore volume of 2.85 cmB.g-1. IMHCSs exhibited a very high loading capacity （1,040 ~tg-mg-1） towards ovalbumin （OVA, a model antigen）, controlled OVA release behavior, excellent safety profile to normal cells, and high antigen delivery efficacy towards macrophages. In vivo immunization studies in mice demonstrated that OVA-loaded IMHCSs induced a 3-fold higher IgG response compared to a traditional adjuvant QuilA used in veterinary vaccine research. OVA delivered by IMHCSs induced a higher IgG1 concentration than IgG2a, indicating a T-helper 2 （Th2）-polarized response. Interferon-y and interleukin-4 concentration analysis revealed both T-helper 1 （Thl） and Th2 immune responses induced by OVA- loaded IMHCSs. IMHCSs are safer adjuvants than QuilA. Our study revealed that pure IMHCSs without further functionalization can be used as a safe adjuvant for promoting Th2-biased immune responses for vaccine delivery.

关 键 词：invaginated mesoporouscarbon hollow spheres ADJUVANT OVALBUMIN vaccine immune response 

分 类 号：S816.7[农业科学—饲料科学] TQ028.8[农业科学—畜牧学]