桃果实向光素基因PpPHOT1和隐花色素基因PpCRY2的克隆与表达分析  

Molecular Cloning and Expression Analysis of PpPHOT1 and PpCRY2 in Postharvest Peach Fruits

作　　者：柴吉钏 梁敏华[1] 郑安然[1] 杨民杰 陈伟[1] 杨震峰[1] 

机构地区：[1]浙江万里学院生物与环境学院,浙江宁波315100

出　　处：《核农学报》2018年第1期30-39,共10页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31371866);浙江省自然科学基金(LQ15C200004)

摘　　要：蓝光是调节植物生长发育最重要的环境因素之一。为了研究蓝光对桃果实类胡萝卜素合成的影响,本试验利用RT-PCR结合RACE的方法,从桃果实中克隆了向光素基因PpPHOT1以及隐花色素基因PpCRY2的c DNA全长序列。序列和生物信息学分析结果表明,PpPHOT1含有向光素所共有的蛋白保守功能域,即N端的LOV结构域和C端的丝/苏氨酸激酶结构域;而PpCRY2则含有隐花色素所共有的蛋白保守功能域,即N端的光裂解酶类似结构域(PHR)和C端的DAS功能域。蓝光处理显著增强了桃果实PpPHOT1和PpCRY2基因的表达,这表明PpPHOT1和PpCRY2可能是桃果实采后响应外源蓝光信号的重要因子。此外,PpPHOT1和PpCRY2在金丽果实中的表达量显著高于湖景蜜露果实,表明蓝光处理诱导金丽类胡萝卜素积累效应更显著,这可能与果实的品种有关。本研究结果为揭示蓝光诱导桃果实类胡萝卜素合成的分子机理提供了一定的理论依据。Blue light is one of the most important environmental factors to regulate plant growth and development. In order to investigate the effect of blue light on carotenoid synthesis of peach fruit,the full-length c DNAs of two blue light photoreceptor genes（ phototropin and cryptochrome） were isolated from peach fruit by RT-PCR and RACE. Sequencing and bioinformatics analyses indicated that PpPHOT1 protein contain two LOV domains in the N-and a serine/threonine protein kinase domain,in the C-termini,which are conserved domains of phototropin,respectively. PpCRY2 protein contained a typical N-terminal photolyase homologous region（ PHR） and a C-terminal DAS motif. The blue light treatment increased the expression of PpPHOT1 and PpCRY2 in both Jinli and hujingmilu fruit. In addition,a significantly higher level of PpPHOT1 and PpCRY2 transcripts in blue light-treated fruit was found in Jinli as compared to that in hujingmilu fruit. These results suggest that PpPHOT1 and PpCRY2 are the important response factors in sensing blue light in peach fruit,especially in Jinli fruit. The results of this study provided some valuable information for further disclosing the regulatory mechanism of blue light on carotenoid synthesis of peach fruit.

关 键 词：桃果实 蓝光 PpPHOT1 PpCRY2 类胡萝卜素 

分 类 号：Q943.2[生物学—植物学] S662.1[农业科学—果树学]