Multimodal bioimaging based on gold nanorod and carbon dot nanohybrids as a novel tool for atherosclerosis detection  

作　　者：Xiaojing Liu Luting Liu Xiujie Hu Shuyun Zhou Rinat Ankri Dror Fixler Zheng Xie 

机构地区：[1]Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China [2]Faculty of Engineering and Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 52900, Israel

出　　处：《Nano Research》2018年第3期1262-1273,共12页纳米研究（英文版）

基　　金：This work was supported by the Joint NSFC-ISF Research Program （No. 51561145004）, jointly funded by the National Natural Science Foundation of China and the Israel Science Foundation, and the President＇s International Fellowship Initiative, Chinese Academy of Sciences （No. PIFI2015VTB041）.

摘　　要：Advanced biocompatible and robust platforms equipped with diverse properties are highly required in biomedical imaging applications for the early detection of atherosclerotic vascular disease and cancers. Designing nanohybrids composed of noble metals and fluorescent materials is a new way to perform multimodal imaging to overcome the limitations of single-modality counterparts. Herein, we propose the novel design of a multimodal contrast agent; namely, an enhanced nanohybrid comprising gold nanorods （GNRs） and carbon dots （CDs） with silica （SiO2） as a bridge. The nanohybrid （GNR@SiO2@CD） construction is based on covalent bonding between SiO2 and the silane-functionalized CDs, which links the GNRs with the CDs to form typical core-shell units. The novel structure not only retains and even highly improves the optical properties of the GNRs and CDs, but also possesses superior imaging performance in both diffusion reflection （DR） and fluorescence lifetime imaging microscopy （FLIM） measurements compared with bare GNRs or fluorescence dyes and CDs. The superior bioimaging properties of the GNR@SiOa@CD nanohybrids were successfully exploited for in vitro DR and FLIM measurements of macrophages within tissue-like phantoms, paving the way toward a theranostic contrast agent for atherosclerosis and cancer.Advanced biocompatible and robust platforms equipped with diverse properties are highly required in biomedical imaging applications for the early detection of atherosclerotic vascular disease and cancers. Designing nanohybrids composed of noble metals and fluorescent materials is a new way to perform multimodal imaging to overcome the limitations of single-modality counterparts. Herein, we propose the novel design of a multimodal contrast agent; namely, an enhanced nanohybrid comprising gold nanorods （GNRs） and carbon dots （CDs） with silica （SiO2） as a bridge. The nanohybrid （GNR@SiO2@CD） construction is based on covalent bonding between SiO2 and the silane-functionalized CDs, which links the GNRs with the CDs to form typical core-shell units. The novel structure not only retains and even highly improves the optical properties of the GNRs and CDs, but also possesses superior imaging performance in both diffusion reflection （DR） and fluorescence lifetime imaging microscopy （FLIM） measurements compared with bare GNRs or fluorescence dyes and CDs. The superior bioimaging properties of the GNR@SiOa@CD nanohybrids were successfully exploited for in vitro DR and FLIM measurements of macrophages within tissue-like phantoms, paving the way toward a theranostic contrast agent for atherosclerosis and cancer.

关 键 词：NANOHYBRIDS multimodal imagingcontrast agent diffusion reflection fluorescence lifetimeimaging ATHEROSCLEROSIS MACROPHAGES 

分 类 号：Q513.5[生物学—生物化学] TD953[矿业工程—选矿]