粪肠球菌LTA通过促进ROS的高表达活化NLRP3炎性体  

Enterococcus Faecalis LTA Activates NLRP3 Inflammasome by Promoting High Expression of ROS

作　　者：王丽娜[1] 叶丹丹[1] 王娇娇[1] 牛卫东[1] 

机构地区：[1]大连医科大学口腔医学院牙体牙髓科,辽宁大连116044

出　　处：《口腔医学研究》2018年第2期112-116,共5页Journal of Oral Science Research

基　　金：国家自然科学基金(81171538)

摘　　要：目的:检测粪肠球菌脂磷壁酸LTA能否通过激活活性氧而活化NLRP3炎性体。方法:粪肠球菌LTA作用于小鼠巨噬细胞RAW264.7,运用Western blot、ELISA及Real-time qPCR法检测NLRP3炎性体蛋白及mRNA的表达。还原型烟酰胺腺嘌呤二核苷酸磷酸酶氧化酶抑制剂DPI抑制活性氧(reactive oxygen species,ROS)的产生,荧光染色及流式细胞仪检测ROS的表达。结果:LTA作用于RAW264.7细胞24小时后,NLRP3、Caspase-1、IL-1β蛋白及mRNA的表达量均明显高于阴性对照组(P<0.05)。DPI可有效抑制ROS的产生,ROS表达被抑制后,NLRP3炎性体蛋白及mRNA的表达亦明显降低。结论:LTA是粪肠球菌活化NLRP3炎性体的毒力因子,主要借助于ROS的产生。Objective：To detect whetherEnterococcus faecalis LTA activates NLRP3 inflammasome by promotingreactive oxygen species expression.Methods：Mouse macrophages RAW264.7 cells were stimulated with Enterococcus faecalis LTA,and the expressions of NLRP3 protein and mRNA were detected by Western blot,ELISA,and real-time qPCR.Reduced nicotinamide adenine dinucleotide phosphatase oxidase inhibitor DPI inhibited the production of reactive oxygen species（ROS）,which was detected by fluorescence staining and flow cytometry.Results：After LTA was applied to RAW264.7 cells for 24 hours,the expressions of protein and mRNA of NLRP3,Caspase-1,and IL-1βwere significantly higher than those of negative control group（P0.05）.The DPI could effectively inhibit the production of ROS.When ROS expression was inhibited,the expressions of NLRP3 inflammatory protein and mRNA were significantly reduced.Conclusion：LTA is the virulence factor of Enterococcus faecalis activating NLRP3 inflammasome,mainly by the production of ROS.

关 键 词：NLRP3炎性体 粪肠球菌 LTA ROS 

分 类 号：R781[医药卫生—口腔医学]