ε-聚赖氨酸生产菌的硫酸二乙酯诱变及其发酵培养基优化  

Diethyl Sulfate Mutagenesis of ε-Poly-L-Lysine Producing Strains and Optimization of Fermentation Medium

作　　者：李聪 扶教龙 施磊 吴晨奇 扶明明 胡翠英 

机构地区：[1]苏州科技大学化学生物与材料工程学院,江苏苏州215009 [2]苏州农业职业技术学院食品科技学院,江苏苏州215008 [3]赣州农业学校,江西赣州341100

出　　处：《中国调味品》2018年第3期34-40,共7页China Condiment

基　　金：江苏省高校自然科学研究面上项目(16KJD550002);苏州市科技计划项目(SYN201410;SYN201507);苏州科技学院科研基金项目(XKZ201411)

摘　　要：对出发菌白色链霉菌FQ-23进行硫酸二乙酯诱变,经过平板初筛和摇瓶复筛后,获得1株ε-聚赖氨酸产量为0.976g/L的高产菌株,并将其命名为白色链霉菌FQF-3。可稳定遗传,并用于后续研究。然后通过单因素与响应面分析方法对4大主要成分:葡萄糖、硫酸铵、玉米浆和鱼粉进行了优化,最终优化结果为:硫酸铵、鱼粉、玉米浆、葡萄糖的含量分别为9.95,12.52,7.79,49.70g/L。再加上M3G培养基的剩余成分即为最适培养基。在该培养基下,ε-聚赖氨酸产量为1.373g/L,是出发菌产量的1.53倍。Streptomyces albulus FQ-23 is mutagenesised by diethyl sulfate in this study. After flat screening and shake flask screening, a high-yielding strain with an ε-poly-L-lysine yield of 0. 976 g/L is obtained. It is named as Streptomyces albulus FQF-3. It could be stably hereditary and used for follow-up study. Four major components： glucose, （NH4）2SO4, corn steep liquor and fish meal are optimized by single factor experiment and response surface methodology. The final optimization results are （NH4）2SO4 of 9.95 g/L,fish meal of 12.52 g/L,corn steep liquor of 7.79 g/L,glucose of 49.70 g/L, and the remaining component of M3G medium is the most suitable medium. Under this medium, the production of ε-poly-L-lysine is 1. 373 g/L,which is 1.53 times higher than that of FQ-23.

关 键 词：白色链霉菌 Ε-聚赖氨酸 硫酸二乙酯诱变 响应面分析法 

分 类 号：TS201.3[轻工技术与工程—食品科学]