蓝莓叶多酚单体化合物与甲醛反应特性研究  

Studies on Reaction Characteristics of Individual Phenolic Compounds from Blueberry Leaf' Polyphenols with Formaldehyde

作　　者：李颖畅 王亚丽 李学鹏 朱学文 韩美洲 励建荣 

机构地区：[1]渤海大学食品科学与工程学院辽宁省食品安全重点实验室生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013

出　　处：《中国食品学报》2018年第2期71-78,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31201308);"十二五"国家科技支撑计划项目(2012BAD29B06)

摘　　要：通过对蓝莓叶多酚单体化合物——绿原酸、槲皮素-3-D-半乳糖苷与甲醛反应特性研究,探讨绿原酸、槲皮素-3-D-半乳糖苷作为甲醛捕获剂的可能性。研究绿原酸、槲皮素-3-D-半乳糖苷浓度、反应温度、反应时间、pH对甲醛清除率的影响,并对反应产物进行高效液相色谱(HPLC)、UV、傅里叶红外光谱扫描分析,从而阐明绿原酸、槲皮素-3-D-半乳糖苷清除甲醛的机理。结果表明:绿原酸、槲皮素-3-D-半乳糖苷浓度、反应温度、反应时间、pH对甲醛清除率均有显著(P<0.05)影响;随着多酚单体浓度、反应时间的增加,甲醛清除率提高;随着温度的增加,甲醛清除率也随之提高,温度达到100℃后甲醛清除率略有下降,说明较高的反应温度、浓度,延长加热时间有利于提高甲醛清除率;当绿原酸、槲皮素-3-D-半乳糖苷pH分别为5和8时与甲醛反应活性最高。对反应产物鉴定分析,有新脂类物质生成,说明绿原酸、槲皮素-3-D-半乳糖苷清除甲醛的机理是发生酚醛缩合反应。The reaction characteristics of individual phenolic componds（5-O-Caffeoylquinic acid and quercetin-3-Dgalactoside） from blueberry leaf polyphenols with formaldehyde were studied to explore the possibility of individual phenolic componds as formaldehyde capture agent. Effects of 5-O-Caffeoylquinic acid and quercetin-3-D-galactoside concentration, reaction temperature, reaction time, p H value were examined on the removal rate of formaldehyde. The reaction products were analyzed by HPLC, UV, Fourier transform infrared（FTIR） spectroscopy. The results showed that effects of concentration, heating temperature, heating time, p H value on the removal rate of formaldehyde were remarkable. The removal rate of formaldehyde increased with the increase of concentration and reaction time. The removal rate of formaldehyde increases with the increase of the temperature, and the removal rate of formaldehyde is decreased slightly after 100 ℃. Therefore, the higher reaction time and concentration and the longer reaction time were in favor of the increase of formaldehyde clearance. When the p H value was five and eight respectively, the reactivities of 5-O-Caffeoylquinic acid and quercetin-3-D-galactoside with formaldehyde were the highest. There were the appearance of new functional groups, indicating that the new compounds. The mechanism that 5-O-Caffeoylquinic acid and quercetin-3-Dgalactoside can react with formaldehyde was phenolic aldehyde condensation reaction.

关 键 词：蓝莓叶多酚 绿原酸 槲皮素-3-D-半乳糖苷 甲醛清除率 酚醛缩合反应 

分 类 号：TS201.2[轻工技术与工程—食品科学]