生食三文鱼中副溶血性弧菌污染调查及耐药性分析  

Infection Survey and Drug Resistance of Vibrio parahaemolyticus in Raw Atlantic Salmon

作　　者：古小莉[1] 李惠青 吕晓瑜[1] 李永贤[1] 杨宏亮[1] 黄珂[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,农业部水产品贮藏保鲜质量安全风险评估实验室,广东广州510300

出　　处：《水产科学》2018年第2期267-270,共4页Fisheries Science

基　　金：中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助项目(2015TS18); 广东省水产品质量安全专项项目(GDSCZA2015009)

摘　　要：探究了广州市售生食三文鱼中副溶血性弧菌的污染状况及分离株的耐药性,为生食三文鱼的食用安全及食源性疾病的防控提供依据。2016年在广州市的批发市场、农贸市场和超市共采集生食三文鱼65份,副溶血性弧菌的分离按照GB 4789.7—2013方法,采用全自动微生物鉴定仪进行副溶血性弧菌的鉴定及药敏试验。2016年广州市生食三文鱼副溶血性弧菌总体检出率为35.38%。7月副溶血性弧菌检出率最高(50%),10月次之,最低为1月。批发市场、农贸市场和超市的副溶血性弧菌检出率分别为45%,9%、9%,三者间有极显著差异(P<0.01)。从65份样品中分离得到63株副溶血性弧菌,分离株的药敏分析发现,所有分离株对阿莫西林、头孢他啶、头孢曲松、头孢吡肟、氨曲南、亚胺培南、美罗培南、阿米卡星、庆大霉素、环丙沙星、左氧氟沙星和呋喃妥因等12种抗生素敏感,少数菌株对复方新诺明耐药(6.35%),大多数菌株对氨苄西林耐药(98.41%)。广州市售生食三文鱼中副溶血性弧菌污染较严重,分离的副溶血性弧菌存在一定程度的耐药情况,今后应加强对生食三文鱼中副溶血性弧菌的监测和抗生素的使用管理。The isolation,identification and drug resistance of pathogen Vibrio parahaemolyticus were carried out in 65 samples of edible raw Atlantic salmon（Salmo salar）collected from wholesale markets,traditional markets and supermarkets in Guangzhou in 2016 according to GB 4789.7—2013 method and by automatic microbial identification system to evaluate the contamination status and drug resistance of V.parahaemolyticus in edible raw salmon in Guangzhou,and to provide references for food safety of edible raw salmon and prevention and control of foodborne diseases.It was found that the detection rate of V.parahaemolyticus was 35.38%（23/65）in edible raw salmon in Guangzhou in 2016,the peak detection rate（50%）in July,followed by in October,and the minimal in January.The detection rate was 45%in wholesale markets,9%in traditional markets and 9% in supermarkets,with very significant difference among different sample sites（P0.01）.The drug sensitive test revealed that 63 strains of V.parahaemolyticus isolated from 65 samples showed highly sensitive to 12 antibiotics such as amoxicillin,ceftriaxone,ceftazidime,cefepime,aztreonam,imipenem,meropenem,amikacin,gentamicin,ciprofloxacin,levofloxacin and nitrofurantoin.A few strains（6.35%）had resistant to co-trimoxazole,and most of the strains（98.41%）generally resistant to ampicillin.The edible raw salmons from Guangzhou markets were highly contaminated by V.parahaemolyticus and the isolated strains had drug resistance to a certain extent,indicating that it was necessary to strengthen monitoring of V.parahaemolyticus in raw edible salmons and management of antibiotics usage in future.

关 键 词：三文鱼 副溶血性弧菌 污染 耐药性 

分 类 号：S917[农业科学—水产科学]