乳酸克鲁维酵母Kluyveromyces lactics整细胞催化苯乙酮制备(R)-1-苯乙醇工艺研究  

Study of Process of Whole Cell Catalysis of Acetophenone to(R)-1-Phenyl Ethanol by Kluyveromyces lactics

作　　者：曹纲 郑美娟[1] 郭金玲[1] 田毅红[1] 吕育财[1] 余华顺[2] 姚鹃[2] 龚大春[1] Cao Gang, Zheng Meijuan, Lv Yucai, Yu Huashun2(1. Biocatalysis Key Laboratory, China Three Gorges Ltd. , Yichang 443002, China） Guo Jinling~ Tian Yihong~ Yao Juan2 Gong Dachun1 Univ., Yichang 443002, China; 2. Angel Yeast Co., Ltd. , Yichang 443002, Chin)

机构地区：[1]三峡大学生物催化重点实验室,湖北宜昌443002 [2]安琪酵母股份有限公司酶制剂事业部,湖北宜昌443002

出　　处：《三峡大学学报（自然科学版）》2018年第2期108-112,共5页Journal of China Three Gorges University：Natural Sciences

基　　金：国家自然基金面上项目"近平滑假丝酵母羰基还原用酶的定点突变及其催化特性研究"(21076114)

摘　　要：在研究乳酸克鲁维酵母(Kluyveromyces lactics)生长特性基础上,确定了该菌株最佳碳源和用量为蔗糖23.8g/L,最佳氮源和用量为酵母浸粉10.0g/L;研究发现,与静息细胞催化相比,乳酸克鲁维酵母(Kluyveromyces lactics)在培养18h后添加底物苯乙酮催化更加有利于转化为(R)-1-苯乙醇;最后对苯乙酮催化工艺进行了5因素4水平的正交实验优化.结果表明,Kluyveromyces lactics细胞采用蔗糖23.0g/L,酵母浸粉10.0g/L,磷酸二氢钾2.0g/L,七水合硫酸镁1.5g/L,30℃条件下培养18h后,每隔4h分3次添加底物苯乙酮,底物容量为249mmol/L,整细胞催化24h,(R)-1-苯乙醇化学收率95%,光学收率均达到99.5%以上.The optimal carbon and nitrogen sources and usages were determined on the base of the research of the Kluyveromyces lactics growth, respectively with the sucrose 23.8 g / L and the yeast extract powder 10. 0 g/L. The Kluyveromyces lactics cell after culturing 18 hrs was better for Acetophenone to （R）-l-phenyl ethanol compared with the resting cell. Finally, the orthogonal test with L16 （45） was performed for the opti- mization of the biocatalytical process with the whole cell. The results show that the chemical yield of the ace- tophenone to（R）-l-phenyl ethanol can be up to 95 %, the optical yield of（R）-l-phenyl ethanol can be up to 99.5% after 24 hrs of the bioconversion with the substrate concentration of the acetophenone 249 mmol/L by three times addition when the Kluyveromyces lactics cell was cultured 18hrs under the conditions of the su- crose23.0 g / L and the yeast extract powder 10.0 g/L K2HPO4 2.0g/L, MgSO4.TH20 1.5g/L at 30℃.

关 键 词：乳酸克鲁维酵母 整细胞催化 (R)-1-苯乙醇 

分 类 号：Q814[生物学—生物工程]