FBXW7基因点突变对结直肠癌HCT-116细胞的增殖、迁移、侵袭和抗凋亡能力的影响  

The effect of FBXW7 gene point mutation to colorectal cancer HCT-116 cells' proliferation, migration, invasion, and its apoptosis resistance

作　　者：张百川[1] 程勇[1] 王祥峰[2] 唐康[1] 王五艺 ZHANG Baichuan1, CHENG Yong1, WANG Xiangfeng2, TANG Kang1, WANG Wuyi1(1 .Department of Gastrointestinal Surgery, the 1st Hospital Affiliated to Chongqing Medical University, Chongqing 400016, China; 2. Department of General Surgery, Daping Hospital and the Research Institute of Surgery, Third Military Medical University, Chongqing 400016, Chin)

机构地区：[1]重庆医科大学附属第一医院胃肠外科,重庆400016 [2]第三军医大学附属大坪医院普外科,重庆400016

出　　处：《中国肿瘤生物治疗杂志》2018年第3期263-269,共7页Chinese Journal of Cancer Biotherapy

基　　金：重庆市科委自然科学基金项目(No.cstc2012jj A10038)~~

摘　　要：目的:探讨FBXW7基因点突变对结直肠癌细胞株HCT-116增殖、凋亡、迁移和侵袭的影响。方法:通过构建FBXW7基因野生型及突变型过表达的重组载体,并将其转染HCT-116细胞,应用Western blotting检测转染后FBXW7蛋白表达情况。CCK-8检测细胞增殖能力,平板细胞克隆形成实验(HTCA)检测肿瘤细胞克隆形成能力,流式细胞术检测细胞凋亡情况,划痕及Transwell细胞迁移实验检测细胞迁移和侵袭能力。结果:转染野生型FBXW7基因的HCT-116细胞中FBXW7蛋白表达水平高于对照组(转染突变型FBXW7基因的HCT-116细胞)、阴性对照组(转染空质粒载体p EZ-M90的HCT-116细胞)及空白对照组(未进行任何特殊处理的HCT-116细胞)(均P<0.05)。转染野生型FBXW7的HCT-116细胞的增殖、克隆形成、迁移和侵袭能力相较于其余各组均显著下降(均P<0.05),且细胞凋亡率显著升高(P<0.05)。结论:FBXW7基因点突变可使其蛋白表达水平降低,从而促进结直肠癌HCT-116细胞的增殖、迁移和侵袭,并抑制其凋亡。Objective： To investigate the impact of FBXW7 gene mutation on cell proliferation, apoptosis, migration, and invasion processes of colorectal cancer HCT-116 cell line. Methods： Recombinant plasmids carrying wild-type and mutant-type FBXW7 SNP were constructed and transfected into HCT-116 cell line; the FBXW7 protein expression level in HCT-116 strains after transfection was detected by Western blotting. Subsequently, cell proliferation capacity was tested by CCK-8 assay; tumor cell colony formation ability was tested by HTCA; cell apoptosis function was tested by FCM; cell migration and invasion were tested by scratch assay and Transwell assay, respectively. Results： Higher HBXW7 protein expression level was detected in HCT-116 strain transfected with wild-type HBXW7 in comparison to the control group（strains transfected with mutant-type HBXW7）, negative-control（strains transfected with empty plasmids）, and blank-control（strains untransfected）（all P0.05）. Compared with the other groups, strains transfected with wildtype HBXW7 exhibited significantly reduced proliferation, colony formation, migration and invasion ability（all P0.05）, but obviously increased apoptosis rate（P0.05）. Conclusion：FBXW7 gene mutation can down-regulate its protein expression, and further promote the proliferation, migration and invasion as well as inhibit the apoptosis of HCT-116 cells.

关 键 词：FBXW7基因 基因突变 结直肠癌 HCT-116细胞 泛素蛋白酶体系统 

分 类 号：R735.3[医药卫生—肿瘤] R730.5[医药卫生—临床医学]