高效液相色谱法检测乳中β-内酰胺酶抑制剂他唑巴坦  

Determination of β-lactamase inhibitor Tazobactam in milk by high performance liquid chromatography

作　　者：于国萍[1] 范美婧 郭佩佩[1] 陈媛[1] 刘鹏[1] 董良伟 YU Guo-ping, FAN Mei-jing, GUO Pei-pei, CHEN Yuan, LIU Peng, DONG Liang-wei(Food Science College of Northeast Agricultural University, Harbin 150030, Chin)

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品工业科技》2018年第6期204-209,共6页Science and Technology of Food Industry

基　　金："十二五"国家科技支撑计划课题项目(2013BAD18B00)

摘　　要：建立了乳中β-内酰胺酶抑制剂他唑巴坦的高效液相色谱检测方法。样品采用乙腈作为沉淀蛋白溶剂,正己烷作为脱脂溶剂。最佳色谱条件:色谱柱为Hypersil ODS-2 C_(18) (4.6 mm×250 mm,5μm),流动相为A∶B为(10∶90),检测波长为213 nm。结果表明,他唑巴坦在5~200μg/mL的浓度范围内具有良好的线性关系,且相关系数是0.9991,采用空白基质匹配标准溶液的方法进行标准校正,显示他唑巴坦的平均回收率在88.40%~94.17%之间。该方法具有操作简单,灵敏度较高,结果准确等特点,适用于乳中他唑巴坦的检测。An analytical method was developed and verified for the determination of β-lactamase inhibitor Tazobactam in milk by high performance liquid chromatography.The sample was deproteinized by acetonitrile and degreased by n-hexane. The chromatographic conditions was chosen Hypersil ODS-2 C18 chromatographic column using A： B （10： 90）as mobile phases with 213 nm detection wavelength.The results of the calibration curve showed perfect linear in the range of 5-200 μg/mL and the correlation coefficient was 0.9991.Matrix-matched calibration standard was used for the quantification and showed the average recoveries ranges from 88.40% -94.18%. With the advantages of simple operation, high sensitivity and accurate results, the method was suitable for the detection of Tazobactam in milk.

关 键 词：高效液相色谱 β-内酰胺酶抑制 他唑巴坦 乳 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]