脑胶质瘤组织肿瘤浸润性树突状细胞及其分子标志物MHC-Ⅱ与CD54的表达情况及意义  

Status of tumor infiltrating dendritic cells in glioma tissue and the expression levels of MHC-Ⅱ and CD54 in such cells

作　　者：沈罡 沈宏 胡未伟 朱光耀 王波定 孙成丰 SHEN Gang, SHEN Hong, HU Wei-wei, et al(Department of Neurosurgery, Ningbo Lihuili East Hospital, Ningbo, Zhejiang 315040, Chin)

机构地区：[1]宁波市医疗中心李惠利东部医院神经外科,浙江宁波315040 [2]浙江大学医学院附属第二医院神经外科,浙江杭州310009

出　　处：《中华全科医学》2018年第4期552-554,653,共4页Chinese Journal of General Practice

基　　金：浙江省自然科学基金(LY17H16001);宁波市自然基金科技计划项目(2012A610195)

摘　　要：目的探讨脑胶质瘤组织肿瘤浸润性树突状细胞(tumor infiltrating dendritic cells,TIDC)及其分子标志物MHC-Ⅱ、CD54表达情况及意义。方法选择宁波市李惠利医院神经外科2013年1月—2017年6月脑胶质瘤组织标本70例作为脑胶质瘤组织组,正常脑组织标本70例作为正常脑组织组,脑胶质瘤组根据WHO病理分级分为低级别脑胶质瘤组(Ⅰ级+Ⅱ级)19例和高级别脑胶质瘤组(Ⅲ级+Ⅳ级)51例。采用免疫组化法测定脑胶质瘤组织TIDC数量和正常脑组织中树突状细胞(dendritic cells,DC)数量,采用流式细胞仪检测脑胶质瘤组织TIDC和正常脑组织中DC膜表面MHC-Ⅱ、CD54表达。结果细胞核出现棕褐色颗粒为S-100阳性染色的DC细胞。脑胶质瘤组织中TIDC数量低于正常脑组织中DC数量(P<0.05);高级别脑胶质瘤组织中TIDC数量低于低级别脑胶质瘤组织中TIDC数量(P<0.05)。脑胶质瘤组织TIDC膜表面MHC-Ⅱ、CD54表达量低于正常脑组织中DC膜表面MHC-Ⅱ、CD54表达(P<0.05)。高级别脑胶质瘤组织TIDC膜表面MHC-Ⅱ、CD54表达量低于低级别脑胶质瘤组织TIDC膜表面MHC-Ⅱ、CD54表达量(P<0.05)。结论脑胶质瘤组织中TIDC细胞少于正常脑组织中DC细胞,随着脑胶质瘤级别的升高,TIDC细胞浸润数目减少,脑胶质瘤存在抗原提呈功能缺失或降低。Objective To investigate the number of tumor infiltrating dendritic cells in glioma tissue and the expression levels of MHC-Ⅱ and CD54 in such cells. Methods Total 70 cases of glioma tissue specimens were taken as glioma tis- sue group, and 70 cases of normal brain tissue specimens were taken as normal brain tissue group in Ningbo Lihuili Hos- pital from January, 2013 to June, 2017. The glioma group was divided into low grade glioma group （grade Ⅰ ＋ grade Ⅱ , 19 cases） and high grade glioma group （grade Ⅲ ＋ Ⅳ, 51 cases） according to WHO pathological grade. The num- ber of TIDCs in brain glioma tissues and the number of dendritic cells （DCs） in normal brain tissues were measured by immunohistochemistry. The expressions of MHC-Ⅱ and CD54 on the surface of DCs were detected by flow eytometry in TIDC and normal brain tissues. Results The nuclei appear as brown particles were S-100 positive staining DC cells. The number of TIDCs in glioma tissue was lower than that in normal brain tissue （ P 〈 0.05 ）. The number of TlDCs in high-grade gliomas was lower than that in low-grade gliomas （P 〈 0.05）. The expression of MHC-Ⅱ and CD54 on the surface of TIBC was lower than that in normal brain tissue （ P 〈 O. 05 ）. The expression of MHC-Ⅱ and CD54 on the TIDC membrane in high-grade gliomas tissue was higher than that in the low-grade glioma tissue （ P 〈 0.05 ）. Conclusion TIDC cells in brain glioma tissue is less than normal brain tissue DC cells. With the increase of glioma grade, TIDC cell infiltration decreases. The presence of antigenic presentation in the glioma is absent or decreased.

关 键 词：脑胶质瘤 肿瘤浸润性树突状细胞 MHC-Ⅱ CD54 

分 类 号：R739.4[医药卫生—肿瘤] R446.8[医药卫生—临床医学]