UPLC-Quadrupole/Orbitrap HRMS同时测定玉米中多种真菌毒素和农药残留  

Fast Determination of Mycotoxins and Pesticide Residues in Maize by UPLC-Quadrupole/Orbitrap HRMS

作　　者：谢刚 叶金[2] 吴宇[2] 王松雪[2] 王硕[1] Xie Gang1,2 Ye Jin2, Wu Yu2, Wang Songxue2, Wang Shuo1(1.College of Food Engineering and Biotechnology, Tianjin University of Science and Technology,Tianjin 300457;2. Academy of State Administration of Grain, Beijing 10003)

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457 [2]国家粮食局科学研究院,北京100037

出　　处：《中国粮油学报》2018年第3期126-133,共8页Journal of the Chinese Cereals and Oils Association

基　　金：国家重点研发计划(2016YFE0113000)

摘　　要：采用直接提取稀释快速前处理技术,利用超高效液相色谱-四级杆/静电场轨道阱高分辨质谱,建立了玉米中常见的16种真菌毒素和11种农药残留的快速同时分析方法。样品采用乙腈∶水∶乙酸溶液(70∶29∶1,V/V/V)提取,提取液稀释后即可进行仪器测定。以C_(18)色谱柱进行色谱分离,通过全扫描模式进行定量检测。结果表明,16种真菌毒素和11种农药残留在本实验的线性范围内都具有良好的线性关系,相关系数(R^2)大于0.999,高中低三浓度添加水平回收率(n=6)为79.6%~116.9%,相对标准偏差为0.5%~12.2%。方法简单、快速和准确,可满足玉米真菌毒素和农药残留的日常检测和监测工作的需要。A UPLC -Quadrupole/Orbitrap high- resolution mass spectrometric （HRMS）method was used for determination of 16 mycotoxins and 11 pesticides in maize. The sample was extracted by aeetonitrile/water/acetic acid in 70 ： 29 ： 1 （V/V/V） ,then diluted and detected by UPLC - HRMS. The targets were separated on a C18 column and quantified by full scan mode. The result showed a good linearity （ R2 〉 0. 999）, the average recoveries and RSD of three different spiked level （n = 6 ）were 79.6% - 116.9% and 0.5 % - 12.2%, respectively. The method is simple, fast, accurate and suitable for daily monitoring of maize.

关 键 词：真菌毒素 农药残留 高分辨质谱 玉米 

分 类 号：TS207.3[轻工技术与工程—食品科学]