Combined acupuncture and HuangDiSan treatment affects behavior and synaptophysin levels in the hippocampus of senescence-accelerated mouse prone 8 after neural stem cell transplantation  

作　　者：Chun-lei Zhou Lan Zhao Hui-yan Shi Jian-wei Liu liang-wei Shi Bo-hong Kan Zhen Li lian-chun Yu Jing-xian Han 

机构地区：[1]Tianjin First Center Hospital, Tianjin, China [2]First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China [3]Tianjin Key Laboratory of Acupuncture and Moxibustion, Tianjin, China [4]Tianjin University of Traditional Chinese Medicine, Tianjin, China

出　　处：《Neural Regeneration Research》2018年第3期541-548,共8页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81202740 and 81603686;the Natural Science Foundation of Tianjin of China,No.17JCYBJC26200 and 12JCQNJC07400;the Public Health Bureau Science and Technology Foundation of Tianjin of China,No.2014KY15;the Specialized Research Foundation for the Doctoral Program of Higher Education,No.20121210120002

摘　　要：Sanjiao acupuncture and HuangDiSan can promote the proliferation, migration and differentiation of exogenous neural stem cells in senescence-accelerated mouse prone 8 （SAMP8） mice and can improve learning and memory impairment and behavioral function in dementia-model mice. Thus, we sought to determine whether Sanjiao acupuncture and HuangDiSan can elevate the effect of neural stem cell transplantation in Alzheimer’s disease model mice. Sanjiao acupuncture was used to stimulate Danzhong （CV17）, Zhongwan （CV12）,Qihai （CV6）, bilateral Xuehai （SP10） and bilateral Zusanli （ST36） 15 days before and after implantation of neural stem cells （5 × 10^5） into the hippocampal dentate gyrus of SAMP8 mice. Simultaneously, 0.2 mL HuangDiSan, containing Rehmannia Root and Chinese Angelica,was intragastrically administered. Our results demonstrated that compared with mice undergoing neural stem cell transplantation alone,learning ability was significantly improved and synaptophysin mRNA and protein levels were greatly increased in the hippocampus of mice undergoing both Sanjiao acupuncture and intragastric administration of HuangDiSan. We conclude that the combination of Sanjiao acupuncture and HuangDiSan can effectively improve dementia symptoms in mice, and the mechanism of this action might be related to the regulation of synaptophysin expression.Sanjiao acupuncture and HuangDiSan can promote the proliferation, migration and differentiation of exogenous neural stem cells in senescence-accelerated mouse prone 8 （SAMP8） mice and can improve learning and memory impairment and behavioral function in dementia-model mice. Thus, we sought to determine whether Sanjiao acupuncture and HuangDiSan can elevate the effect of neural stem cell transplantation in Alzheimer’s disease model mice. Sanjiao acupuncture was used to stimulate Danzhong （CV17）, Zhongwan （CV12）,Qihai （CV6）, bilateral Xuehai （SP10） and bilateral Zusanli （ST36） 15 days before and after implantation of neural stem cells （5 × 10^5） into the hippocampal dentate gyrus of SAMP8 mice. Simultaneously, 0.2 mL HuangDiSan, containing Rehmannia Root and Chinese Angelica,was intragastrically administered. Our results demonstrated that compared with mice undergoing neural stem cell transplantation alone,learning ability was significantly improved and synaptophysin mRNA and protein levels were greatly increased in the hippocampus of mice undergoing both Sanjiao acupuncture and intragastric administration of HuangDiSan. We conclude that the combination of Sanjiao acupuncture and HuangDiSan can effectively improve dementia symptoms in mice, and the mechanism of this action might be related to the regulation of synaptophysin expression.

关 键 词：nerve regeneration neurons NEURODEGENERATION Alzheimer＇s disease MICROENVIRONMENT Chinese medicine BEHAVIOR neural stem cell transplantation SYNAPTOPHYSIN neural regeneration 

分 类 号：R245[医药卫生—针灸推拿学]