海南黑山羊瘤胃液乳酸菌的筛选鉴定及其应用  

Identification and Application of Lactic Acid Bacteria Isolated from the Rumen Fluid of Hainan Black Goat

作　　者：张万昌[1] 谭海生[1] 杨劲松[1] 鞠雪莉 李晓雷[1] 赵建国 李茂[2] 周汉林[2] Zhang Wanchang;Tan Haisheng;Yang Jinsong;Ju Xueli;Li Xiaolei;Zhao Jianguo;Li Mao;Zhou Hanlin(Hainan University, Haikou 570228, China;Tropical Crops Genetic Resources Research Institute Chinese Academy of Tropical Agriclutural Sciences, Danzhou 5717371, China)

机构地区：[1]海南大学食品学院,海南海口570228 [2]中国热带农业科学院热带作物品种资源研究所,海南儋州571737

出　　处：《海南大学学报（自然科学版）》2018年第1期16-25,共10页Natural Science Journal of Hainan University

基　　金：海南省自然科学基金(314073);国家自然科学基金(31460621)

摘　　要：以海南黑山羊瘤胃液和王草为研究对象,从其胃液中筛选乳酸菌,并将其应用到黑山羊草料的青贮中,经形态学观察和乳酸定性试验,对菌株进行了初步鉴定;此后,在利用16S rDNA基因进行测序分析后,有3株代表性菌株被鉴定到种的水平,其中,HBG11与耐久肠球菌的同源性最高,HBG46与植物乳杆菌的同源性最高,HBG86与干酪乳杆菌的同源性最高.选择1株生长旺盛且产酸速度快的乳酸菌,将其用于王草的青贮发酵,同时设置5组不同处理,以此来测定各组乳酸菌的生长状况以及青贮饲料的品质.结果表明:第V组青贮的效果最好,pH值迅速下降,且好氧细菌的数量很快得到了有效控制,最终pH值为3.83,显著低于对照组(P<0.05);青贮50 d后,第V组王草的营养成分与第I组王草的营养成分差异显著(P<0.05),HBG46菌株对王草青贮饲料的有氧稳定性有一定的促进作用.In the report, the lactic acid bacteria were isolated and identified from the rumen fluid of Hainan black goat, and which were used for the black goat feed silage. The obtained strains were identified elementarily by morphology test and lactic acid qualitative test; based on 16S rDNA gene sequencing, three of them, HBG11, HBG46, and HBG86, were identified as Enterococcus durans, LactobaciUus plantarum, Lactobacillus casei, re- spectively; One lactic acid bacteria, which had a high rate of growing and acid production, was chosen for King grass silage fermentation. Five expereimental groups were designed to analyze the growth condition of lactic acid bacteria and quality of silage. The results indicated that the effects of Group V was best, in which was signifi- cantlyreduced （P 〈 0.05 ） , and final PH is 3.83 ; after 50d, the nutritional component of King grass silage in Group V was changed significantly （P 〈 0.05 ）. So, HBG46 had the ability to prevent pH from rising during aerobic period.

关 键 词：瘤胃液 乳酸菌 16S RDNA序列分析 青贮 王草 

分 类 号：S543.6[农业科学—作物学]