红花黄色素预处理对全脑缺血再灌注大鼠海马CA1区神经元Prohibitin／Caspase3信号通路的影响  

Effects of carthamin yellow preconditioning on the prohibitin/caspase3 signaling pathway in hippocampal CA1 neurons of rats after cerebral ischemia - reperfusion

作　　者：武卿 韩东 胡书群 许铁 WU Qing;HAN Dong;HU Shuqun;XU Tie(School of Nursing, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China;Department of Emergency, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002;Institute of Medical Rescue, Xuzhou Medical University, Xuzhou, Jiangsu 221002)

机构地区：[1]徐州医科大学护理学院,江苏徐州221004 [2]徐州医科大学附属医院急诊科,江苏徐州221002 [3]徐州医科大学医学救援研究所,江苏徐州221002

出　　处：《徐州医科大学学报》2018年第3期151-155,共5页Journal of Xuzhou Medical University

摘　　要：目的研究红花黄色素对大鼠海马CA1区Prohibitin（PHB）／Caspase3信号通路的影响，探讨红花黄色素对缺血性神经元损伤的保护作用的可能机制。方法按照随机数字法将48只SD雄性大鼠随机分为：假手术（Sham）组、缺血再灌注（I／R）组、溶剂对照（Vehicle）组、红花黄色素预处理（SY）组；通过大鼠大脑四动脉结扎建立全脑缺血模型。Vehicle组、SY组于脑缺血前7天开始，经股静脉注射2ml生理盐水或红花黄色素（90ms／kg溶于2ml生理盐水中），每天1次。免疫印迹法检测海马CA1区PHB蛋白含量的变化和Caspase3活化水平的变化；焦油紫染色法观察海马CA1区神经元死亡情况。结果缺血再灌注3h时，与I／R组和Vehicle组相比，SY组大鼠海马CA1区PHB蛋白含量明显升高，而Caspase3的活化水平明显降低，差异有统计学意义（P〈0．05）；缺血再灌注第7天，焦油紫染色法结果显示SY组大鼠海马CA1区的存活神经元数量明显多于I／R组和Vehicle组，差异有统计学意义（P〈0．05）。结论红花黄色素预处理对缺血再灌注引起的海马CA1区神经元损伤有保护作用，这可能与Prohibitin的表达增加和Caspase3的活化水平降低有关。Objective To investigate the effects of carthamin yellow preconditioning on the prohibitin （PHB）/ caspase 3 signaling pathway in the hippocampal CA1 region of rats, and to explore the possible mechanism of carthamin yellow on the protection of ischemic neuronal injure. Methods A total of 48 SD male rats were randomly divided into a sham operation （Sham） group, an ischemia- reperfusion （I/R） group, a solvent control （Vehicle） group and a safflow- er yellow pretreatment group （SY） group. A rat model of global cerebral ischemia - reperfusion was established through cerebral four - artery ligation. Rats in Groups Vehicle and SY were injected with 2 ml normal saline or carthamin yellow （90 mg/kg, in 2 ml normal saline） for 7 days before cerebral ischemia, once a day. The levels of PHB and activities of caspase 3 in the hippocampus CA1 region were detected by Western blotting, and the death of neurons in the hippocam- pus CA1 region was observed by tar violet staining. Results At 3 h after, Compared with Groups I/R and Vehicle, Groups SY produced remarkably increased content of PHB in the hippoeampal CA1 but markedly reduced activities of caspase 3 when ischemia and reperfusion for 3 hours （ P 〈 0.05 ）. On the 7th day of isehemia - reperfusion, the number of surviving neurons in the hippoeampal CA1 of the rats in Group SY was significandy higher than that in Groups/JR and Vehicle （P 〈 0.05 ）. Conclusions Preconditioning treatment of carthamin yellow can protect neuronal injury in the CA1 region of hippocampus induced by ischemia - reperfusion, which may be related to increased prohibitin expression and decreased caspase3 activation levels.

关 键 词：红花黄色素 缺血再灌注 PROHIBITIN CASPASE3 

分 类 号：R741.02[医药卫生—神经病学与精神病学]