榛仁五肽对HUVEC细胞氧化应激损伤的保护作用  

Protective Effect of Hazelnut Pentapeptide on Oxidative Stress Injury in HUVECs

作　　者：王祖浩[1] 郭勇 周美含 刘春雷 方丽 闵伟红[1] WANG Zu-hao;GUO Yong;ZHOU Mei-han;LIU Chun-lei;FANG Li;MIN Wei-hong(College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China;National Engineering Laboratory on Wheat and Corn Further Processing, Changchun 130118, China)

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]小麦和玉米深加工国家工程实验室,吉林长春130118

出　　处：《现代食品科技》2018年第3期1-7,共7页Modern Food Science and Technology

基　　金：国家"863"科技计划项目(2013AA102206)

摘　　要：本文以长白山榛仁五肽Ala-Trp-Asp-Pro-Glu(AWDPE)为研究对象,利用体外化学和细胞实验,研究AWDPE抗氧化活性及对HUVEC细胞氧化应激损伤的保护作用。结果显示:AWDPE浓度为1 mg/mL时对DPPH和ABTS自由基清除率分别达到63.38±2.44%和69.38±1.96%;实验范围内,不同浓度AWDPE对HUVEC细胞无生长抑制作用,亦无生长促进作用;与血管紧张素Ⅱ氧化损伤组相比,AWDPE保护组细胞活力高于AngⅡ损伤组1.51倍(p<0.01);100μg/mL AWDPE保护组细胞内,LDH释放率为27.14±1.16%,为AngⅡ损伤组0.62倍(p<0.01);500μg/mL AWDPE保护组细胞内,MDA含量为3.47±0.28 nmol/mg,为AngⅡ损伤组0.73倍;100μg/mL AWDPE保护组细胞内,CAT含量为2.34±0.11 U/mg,为AngⅡ损伤组1.39倍(p<0.05);500μg/mL AWDPE保护组细胞内,T-SOD含量为23.55±1.26 U/mg,为AngⅡ损伤组2.48倍(p<0.01);500μg/mL AWDPE保护组细胞内,GSH-PX含量为33.9±2.75 nmol/L,为AngⅡ损伤组1.91倍(p<0.01)。上述实验证明AWDPE五肽对HUVECs细胞氧化应激损伤具有显著的保护作用。The antioxidant activity of a pentapeptide Ala-Trp-Asp-Pro-Glu（AWDPE） derivated from hazelnuts of Changbai Mountain was investigated and its protective effect on oxidative stress injury in HUVECs were also evaluateded. The results showed that when, the radical scavenging rates of AWDPE（1 mg/mL） on 1,1-diphenyl-2-picrylhydrazyl（DPPH） and 2,2‘-azinobis-（3-ethylbenzthiazoline-6-sulfonic acid）（ABTS） were 63.38 ± 2.44% and 69.38 ± 1.96%, respectively. Different concentrations of AWDPE had neither inhibitory nor promoting effects on the proliferation of HUVECs. HUVEC cell activity of AWDPE protection group was 1.51 times higher in than that of the angiotensin Ⅱ（Ang Ⅱ） injuried group（p 0.01）. The release rate of lactate dehydrogenase（LDH） in 100 μg/mL AWDPE protection group was 27.14 ± 1.16%, which was 0.62 times higher than that of the Ang Ⅱ injuried group（p 0.01）. The methane dicarboxylic aldehyde（MDA） content in 500 μg/mL AWDPE protection group was 3.47 ± 0.28 nmol/mg, which was 0.73 times higher than that of Ang Ⅱ injuried group. The content of catalase（CAT） in 100 μg/mL AWDPE protection group was 2.34 ± 0.11 U/mg, 1.39 times higher than that of the Ang Ⅱ injuried group（p 0.05）. The total superoxide dismutase（T-SOD） content in 500 μg/mL AWDPE protection group was 23.55 ± 1.26 U/mg,2.48 times higher than that of the Ang Ⅱ injuried group（p 0.01）. The glutathione peroxidase（GSH-PX） content was 33.9 ± 2.75 nmol/L in 500 μg/mL AWDPE protection group, which was 1.91 times higher than that of Ang Ⅱ injuried group（p 0.01）. These results indicated that hazelnut pentapeptide AWDPE had significant protective effect against oxidative stress damage in HUVECs.

关 键 词：榛仁肽 血管紧张素Ⅱ 人脐静脉内皮细胞 氧化应激 

分 类 号：TS201.4[轻工技术与工程—食品科学]