大黄鱼FST基因SNP筛选及与生长性状相关性分析  

SNP Detection of FST Gene and Its Association With Growth Traits in Larimichthys crocea

作　　者：张玉[1] 韩明星[1] 曹明月[1] 赵亚运 李娟[1] 薛良义[1] ZHANG Yu;HAN Mingxing;CAO Mingyue;ZHAO Yayun;LI Juan;XUE Liangyi(College of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211)

机构地区：[1]宁波大学海洋学院,浙江宁波315211

出　　处：《核农学报》2018年第5期883-891,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31172398);浙江省育种专项(2016C02055-7);宁波市重大专项(2015C110005)

摘　　要：为探索FST基因单核苷酸多态性(SNP)与大黄鱼生长性状的相关性,本研究采用直接测序和高分辨率熔解曲线(HRM)法分别进行SNP位点筛查和验证分型。结果共筛查到19个SNP位点,其中2个在外显子1,8个在外显子2,7个在外显子3,外显子4和外显子5各1个,近端启动子区域未筛查到突变。位于外显子4的g.2169A>C位点,AA基因型占96.8%,CC基因型占3.2%,多态信息含量为0.0600;位于外显子5的g.2998A>G位点,AA基因型占63.2%,AG基因型占30%,GG基因型占6.8%,多态信息含量为0.2828,这2个位点均处于哈代-温伯格平衡状态。相关性分析表明,在g.2169A>C位点,CC型个体各个生长性状(体重、体长、全长、体高)均大于AA型个体,但差异不显著(P>0.05);g.2998A>G位点,AG型个体各性状均值最大,GG型个体最小,但只在全长性状上基因型AG与GG存在显著性差异(P<0.05),表明FST基因编码区的突变对大黄鱼的生长性状有一定的影响。本试验结果为大黄鱼育种中体型的选择提供了一定的理论依据。In order to explore the correlation between single nucleotide polymorphism（ SNP） of FST gene and growth traits of Larimichthys crocea,an important marine-cultivated fish in China,SNP sites were screened and identified by direct sequencing and high resolution melt（ HRM） in this study. The results showed that a total of 19 SNP sites were obtained,among which 2 in exon 1,8 in exon 2,7 in exon 3,1 in exon 4 and 1 in exon 5. However,no SNP site was obtained in the proximal promoter region. At the g. 2169 A C site located in exon 4,genotype frequencies of two genotypes AA and CC were 0. 968 and 0. 032,respectively,and polymorphic information content（ PIC） was 0. 0600.At the g. 2998 A G site located in exon 5,genotype frequencies of three genotypes AA,AG and GG were 0. 632,0. 300 and 0. 068,respectively,and PIC was 0. 2828. Both two sites were in Hardy-Weinberg equilibrium（ P 0. 05）.The association analysis showed that individuals with genotype CC had higher average values than those individuals with genotype AA in the growth traits（ body weight,body length,overall length and body height） at the g. 2169 A C site,but the difference was not significant（ P 0. 05）. At the g. 2998 A G site,the average value of each growth trait was highest in the individuals of genotype AG,and lowest in the individuals with genotypes GG. However,only overall length between the individuals with genotype AG and GG had significant difference（ P 〈0. 05）. These results imply that the mutations in the coding region of FST gene may have a certain effect on the growth traits in Larimichthys crocea.This result provides the basis for the choice of body shape in the breeding of Larimichthys crocea.

关 键 词：大黄鱼 卵泡抑素 高分辨率熔解曲线 单核苷酸多态性 生长性状 

分 类 号：S917.4[农业科学—水产科学]