熄风胶囊对难治性癫痫大鼠海马Ⅰ型钠通道α亚基蛋白及mRNA表达的影响  

Effects of XiFeng Capsules on α Subunit Protein and mRNA Expressions of Type Ⅰ Sodium Channel in the Hippocam pi of the Rats Suffering Intractable Epilepsy

作　　者：房艳艳[1] 李新民[2] 路岩莉[2] 韩耀巍[2] 聂坤[2] 吴超[1] FANG Yanyan;LI Xinmin;LU Yanli;HAN Yaowei;NIE Kun;WU Chao(Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;First Teaching Hospital of Tianjin University of TCM)

机构地区：[1]天津中医药大学,天津300193 [2]天津中医药大学第一附属医院

出　　处：《西部中医药》2018年第4期14-19,共6页Western Journal of Traditional Chinese Medicine

基　　金：国家自然基金(编号81373690);天津市高等学科科技发展基金计划项目(编号20120214)

摘　　要：目的:探讨熄风胶囊对氯化锂—匹罗卡品致难治性癫痫模型大鼠Ⅰ型钠通道α亚基蛋白及m RNA表达的影响。方法:建立氯化锂—匹罗卡品癫痫大鼠模型。实验大鼠随机分为8组:正常对照组(空白组)、模型对照组(模型组)、熄风胶囊低剂量组(熄低组)、熄风胶囊中剂量组(熄中组)、熄风胶囊高剂量组(熄高组)、卡马西平治疗组(CBZ组)、熄风胶囊中剂量+卡马西平组(熄卡组)、熄风胶囊中剂量+1/2卡马西平组(熄卡低组)。熄低、中、高组分别予熄风胶囊0.33 g、0.66 g、0.99 g,浓缩剂2 m L;CBZ组予CBZ20 mg/kg;熄卡、熄卡低组分别予熄风胶囊0.66 g和CBZ 20 mg/kg、CBZ 10 mg/kg;模型组和空白组分别予生理盐水2 m L。每天上午灌胃1次,共持续60天。给药结束后检测各组大鼠Ⅰ型钠通道α亚基蛋白及m RNA的表达。结果:1)免疫组化染色法示:与空白组比较,模型组SCN1A的表达高于空白组(P<0.05);与模型组比较,各组治疗SCN1A的表达均低于模型组(P<0.05);与CBZ组相比,熄卡组SCN1A的表达均低于CBZ组(P<0.05);2)Westernblot示:与空白组比较,模型组SCN1A的表达高于空白组(P<0.05);与模型组比较,各治疗组SCN1A的表达均低于模型组(P<0.05);与CBZ组比较,熄卡低组SCN1A的表达低于CBZ组(P<0.05);3)Real-time RT-PCR示:熄高组、熄中组、熄低组、熄卡组对SCN1A m RNA表达有抑制作用,而卡马组、熄卡低组对SCN1A m RNA表达有促进作用。结论:免疫组化、Western-blot、Real-time RT-PCR结果显示:与正常大鼠相比,IE大鼠海马SCN1A在蛋白与m RNA两个层面均表达上调。熄风胶囊可能通过抑制癫痫大鼠海马Ⅰ型钠通道α亚基蛋白及基因的表达抑制钠电流,从而降低癫痫反复自发性发作的可能。Objective： To discuss the effects of Xi Feng capsules on α subunit protein and m RNA expressions of type I sodium channel in the hippocampi of the epileptic rats induced by lithium chloride-pilocarpine. Methods：Lithium chloride-pilocarpine induced epileptic rat model was established. The rats were randomized into eight groups： normal control group（the blank group）, model control group（the model group）, low, moderate and high dose groups of Xi Feng capsules（low XF group, moderate XF group and high XF group）, the treatment group of carbamazepine（CBZ group）, moderate dose group of Xi Feng capsules and carbamazepine（XF＋CBZ group） as well as moderate dose group of Xi Feng capsules and 1/2 carbamazepine（XF＋ low CBZ group）, the groups of low XF,moderate XF and high XF were given Xi Feng capsules respectively, 0.33 g, 0.66 g and 0.99 g, inspissant 2 m L; CBZ group accepted CBZ 20 mg/kg; XF＋CBZ group and XF＋ low CBZ group received Xi Feng capsules, 0.66 g, and CBZ 20 mg/kg, CBZ 10 mg/kg; the model group and the blank group were given physiological saline respectively,2 m L, lavage once in the morning each day, for 60 days consecutively. The expressions of m RNA and α subunit protein of type I sodium channel of the rats in different groups were detected after the administration. Results：1） Immunohistochemical staining showed： compared with the blank group, the expressions of SCN1 A in the model group were higher than these of the blank group（P 〈0.05）; compared with the model group, the groups of low XF,moderate XF and high XF, XF＋CBZ group and XF＋ low CBZ group were lower than the model group in the expressions of SCN1 A（P〈0.05）; when different treatment groups were compared with CBZ group, the groups of low XF, moderate XF and high XF were lower than CBZ group in the expressions of SCN1 A（P〈0.05）; 2） Westernblot demonstrated： compared with the blank group, the model group was higher than the blank group in the expressions of SCN1 A（P〈0.05）, the

关 键 词：难治性癫痫 SCN1A 熄风胶囊 Ⅰ型钠通道 α亚基蛋白 

分 类 号：R742.1[医药卫生—神经病学与精神病学]