高效液相色谱法测定虫草胶囊中腺苷的含量  

Determination of content of adenosine in cordyceps capsules by high performance liquid chromatography

作　　者：王桂英 孙伟红[2] 庄燕 WANG Gui-Ying;SUN Wei-Hong;ZHUANG Yan(Jinan Food and Drug Inspection Center, Jinan 250102, China;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Laboratory of Quality ＆ Safety Risk Assessment for Aquatic Products （Qingdao）, Ministry of Agriculture, Qingdao 266071, China)

机构地区：[1]济南市食品药品检验检测中心,济南250102 [2]中国水产科学研究院黄海水产研究所农业部水产品质量安全检测与评价重点实验室农业部水产品质量安全风险评估实验室(青岛),青岛266071

出　　处：《食品安全质量检测学报》2018年第7期1644-1648,共5页Journal of Food Safety and Quality

摘　　要：目的建立高效液相色谱法(high performance liquid chromatography,HPLC)测定虫草胶囊中腺苷的含量。方法样品经过研碎、超声处理、离心、过滤后上机待测。使用岛津VP-ODS(250 mm×4.6 mm,5μm)色谱柱,以甲醇-0.04 mol/L磷酸二氢钾(15:85,V:V)为流动相;流速:1.0 m L/min;进样量:10μL;检测波长为262 nm。结果腺苷在0.7~70μg/m L的范围内呈良好线性关系,r=0.9999,回收率为99.1%~99.5%,相对标准偏差为0.2%~0.6%。结论本研究建立的方法简便、快捷、准确,可准确测定虫草胶囊中腺苷的含量。Objective To establish a method for determining the content of adenosine in cordyceps capsules by high performance liquid chromatography（HPLC）. Methods After crushing, ultrasonic treatment, centrifugation and filtration, samples were detected by HPLC. The C18 column（4.6 mm×250 mm, 5 μm） was used and methanol-0.04 mol/L potassium dihydrogen phosphate solution（15：85, V：V） was used as the mobile phase. The flow rate was 1.0 m L/min and sampling amount was 10 μL. The detection wavelength was 262 nm. Results The calibration curve was linear in the range of 0.7-70 μg/m L for adenosine. The average recovery were 99.1%-99.5% and relative standard deviations（RSD） were 0.2%-0.6%. Conclusion The established method is simple, rapid and accurate, which can be used for the quality control of adenosine in cordyceps capsules.

关 键 词：高效液相色谱法 虫草胶囊 腺苷 

分 类 号：O657.72[理学—分析化学]