大肠杆菌生产莽草酸发酵培养基优化  

Medium optimization for production of shikimate by Escherichia coli

作　　者：韩洪军 张顺棠 高立栋 李燕军 陈宁[2,3] HAN Hongjun;ZHANG Shuntang;GA;LI Yanjun;CHEN Ning(Post-Doctoral Research Center, Lotus Health Group Co. , Ltd. , Xiangcheng 466200, China;College of Biotechnology,Tianjin. University of Science and Technology, Tianjin. 300457, China;National and Local United Engineering Lab of Metabolic Control Fermentation. Technology, Tianjin 300457, China)

机构地区：[1]莲花健康产业集团股份有限公司博士后科研工作站,河南项城466200 [2]天津科技大学生物工程学院,天津300457 [3]代谢控制发酵技术国家地方联合工程实验室,天津300457

出　　处：《发酵科技通讯》2018年第1期33-37,共5页Bulletin of Fermentation Science and Technology

基　　金：中国博士后科学基金资助项目(2016M601269)

摘　　要：利用单因素实验对大肠杆菌莽草酸发酵培养基中碳氮源与金属离子进行了优化,选取对菌体生长和莽草酸产量影响较大的蛋白胨、牛肉膏、FeSO_4和MgSO_4四个因素进行正交实验优化,确定了莽草酸发酵的最适培养基配方:葡萄糖15g/L,蛋白胨10g/L,牛肉膏3g/L,酵母粉2g/L,柠檬酸2g/L,(NH_4)_2SO_41.6g/L,K_2HPO_4·3H_2O 7.5g/L,MgSO_4·7H_2O 2mg/L,FeSO_4·7H_2O 2mg/L,钼酸铵0.009mg/L,硼酸0.17mg/L,CoCl_2·6H_2O 0.047mg/L,MnSO_4·H_2O0.017mg/L,CuSO_4·7H_2O 0.017mg/L,ZnSO_4·7H_2O 0.02mg/L.采用优化后培养基发酵,莽草酸产量达到4.675g/L,为优化前的3.22倍.The carbon sources,nitrogen sources and metal ions in the medium of shikimate fermentation by Escherichia coli were optimized by single factor experiments.Four critical factors including peptone,beef extract,FeSO4·7H2O and MgSO4·7H2O were selected for further optimization using the orthogonal experiment.The optimal medium of shikimate fermentation was determined as follows：glucose 15g/L,peptone 10g/L,beef extract 3g/L,yeast powder 2g/L,citric acid 2g/L,（NH4）2SO41.6g/L,K2HPO4·3H2O 7.5g/L,MgSO4·7H2O 2mg/L,FeSO4·7H2O 2mg/L,ammonium molybdate 0.009mg/L,boric acid 0.17mg/L,CoCl2·6H2O 0.047 mg/L,MnSO4·H2O 0.017 mg/L,CuSO4·7H2O 0.017 mg/L and ZnSO4·7H2O 0.02 mg/L.Under the optimized medium,the shikimate production reached 4.675g/L,which was 3.22times as high as that obtained with the original medium.

关 键 词：大肠杆菌 莽草酸 单因素实验 正交实验 

分 类 号：TQ92[轻工技术与工程—发酵工程]