反相HPLC-ICP-MS法同步检测食品中常见食品添加剂及重金属  

Simultaneous Determination of Common Food Additives and Heavy Metals in Food by Reversed-Phase HPLC-ICP-MS

作　　者：王佳琪 郑锡瀚[1,2,3] 罗萌婕 蓝丽红 WANG Jia-qi;ZHENG Xi-han;LUO Meng-jie;LAN Li-hong(School of Chemistry and Chemical Engineering;Cultivation Base of Guangxi Polysaccharide Material and Modified Key Laboratory;2. Key Laboratory of Chemical and Biological Transformation Process of Higher Education Institutes, Guangxi University for Nationalities, Nanning 530006,China)

机构地区：[1]广西民族大学化学化工学院,广西南宁530006 [2]广西民族大学广西多糖材料与改性重点实验室培育基地,广西南宁530006 [3]广西民族大学广西高校化学与生物转化工程技术重点实验室,广西南宁530006

出　　处：《广西民族大学学报（自然科学版）》2018年第1期90-94,共5页Journal of Guangxi Minzu University ：Natural Science Edition

基　　金：广西科学研究与技术开发--科技创新能力与条件建设计划项目(桂科能14123006-29);广西民族大学大学生创新创业训练计划项目(201710608084)

摘　　要：采用反相高效液相色谱(RHPLC)和电感耦合等离子体质谱(ICP-MS)联用同步检测食品中常见食品添加剂糖精钠、苯甲酸钠、日落黄、山梨酸钾及重金属铅和砷.实验选用ZORBAX Eclipse XDB-C18 Analytical 4.6×250 nm5-Micron为的反向色谱柱;流动相为体积比为10∶90的甲醇和乙酸铵(浓度为0.02 mol/L)混合溶液,在UV254 mn处检测,经前处理的食品样品在液相色谱中分离后检测出食品添加剂含量,进入电感等离子体质谱检测重金属含量.结果表明:食品添加剂检出限为6~15 ng/L,相对标准偏差RSD<3.5%;加样回收率在94%~105%之间;重金属检出限为7~34 ng/L,相对标准偏差RSD<3.0%;加样回收率在90%~97%之间.In that paper,simultaneous determination of sodium saccharin,sodium benzoate,sunset yellow,sorbic acid,heavy metal lead and arsenic in beverages by RP-HPLC-ICP-MS was reported.ZORBAX Eclipse XDB-C18（Analytical 4.6×250 nm 5-Micro）was used as the reverse chromatographic column,with a mixture of methanol/ammonium acetate（0.02 mol/L）（10∶90,V/V）as the mobile phase,and the detection at UV 254 mn.Food additive contents were detected by liquid chromatography after separation of pre-treated food samples,and heavy metal contents were detected by ICP-MS.The results showed that the detection limit of food additives was 6.0~15 ng/L,with less than 4% of relative standard deviation（RSD）,and the addition recovery rate was between 93%~109% The detection limit of heavy metals was 7.0~12 ng/L,with RSD 3.5%,and the addition recovery rate was between 93%~104%.

关 键 词：反相高效液相色谱 电感耦合等离子体质谱 同步检测 食品添加剂 重金属 

分 类 号：TS207.3[轻工技术与工程—食品科学]